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腐食酪螨线粒体基因组全序列(蜱螨目:真螨目),包含 22 个 tRNA 序列和最大的非编码区。

De novo sequence of the mitochondrial genome of Tyrophagus putrescentiae (Acari: Sarcoptiformes) including 22 tRNA sequences and the largest non-coding region.

机构信息

Department of Health Inspection and Quarantine, Wannan Medical College, Wuhu, 241002, China.

Department of Pathology, Wannan Medical College, Wuhu, China.

出版信息

Exp Appl Acarol. 2020 Apr;80(4):521-530. doi: 10.1007/s10493-020-00477-3. Epub 2020 Mar 11.

DOI:10.1007/s10493-020-00477-3
PMID:32162137
Abstract

In this study, we de novo sequenced and analyzed the circular mitochondrial genome (mitogenome) of Tyrophagus putrescentiae. It was 14,156 bp long and contained a complete set of 37 genes, contrary to the initial published sequences; it included 22 tRNA sequences and the largest non-coding region. The mtDNA gene order of T. putrescentiae was found to be identical to that of Aleuroglyphus ovatus, Caloglyphus berlesei, and Rhizoglyphus robini (all Acaroidea). Most tRNAs of T. putrescentiae lack at least a D-arm or T-arm. Tyrophagus putrescentiae tRNAs also shared considerable structural and sequence similarity with the tRNAs of other reported Acaroidea species that have the full set of tRNAs. The largest non-coding region was located between trnF and trnS1, and it contained a microsatellite-like (AT) sequence, short palindromic sequences, and several hairpin loops, as observed in other reported Acaroidea species (excepting Tyrophagus longior).

摘要

在这项研究中,我们从头测序并分析了腐食酪螨(Tyrophagus putrescentiae)的圆形线粒体基因组(mitogenome)。它长 14156bp,包含一整套 37 个基因,与最初公布的序列相反;它包含 22 个 tRNA 序列和最大的非编码区。腐食酪螨的 mtDNA 基因排列与粉虱外崎蚜小蜂(Aleuroglyphus ovatus)、柏小爪螨(Caloglyphus berlesei)和罗宾氏脊胸叶蝉(Rhizoglyphus robini)(均为 Acaroidea)相同。大多数腐食酪螨的 tRNA 至少缺少 D 臂或 T 臂。腐食酪螨的 tRNA 也与其他报道的具有完整 tRNA 组的 Acaroidea 物种的 tRNA 具有相当大的结构和序列相似性。最大的非编码区位于 trnF 和 trnS1 之间,它包含微卫星样(AT)序列、短回文序列和几个发夹环,如在其他报道的 Acaroidea 物种(除了长须螨 Tyrophagus longior)中观察到的那样。

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