Szulc B, Piasecki E
Laboratory of Tumor Virology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.
Arch Immunol Ther Exp (Warsz). 1988;36(5):537-45.
Sodium salt of 9-oxo-10-acridineacetohydroxamic acid (HCA), a new synthetic compound, forms small crystals in aqueous solution. These crystals were easily phagocytized by the mouse bone marrow-derived macrophages. The ingested HCA crystals were visible under light microscope as dark granules. The degree of phagocytosis was estimated by the spectroscopic measurements of absorption of ingested HCA. About 10 day-old cultures of mouse bone marrow-derived macrophages were found to be suitable for a study on the effect of murine or human interferons. It was observed that murine interferons alpha, beta and gamma at low concentrations (10-200 U/ml) stimulated and higher concentrations (400-1000 U/ml) had no effect on the phagocytosis. Previous treatment of interferons with anti-IFN sera abolished the effect of the interferons. CMA-induced interferon and growth factors were found to modify the phagocytic activity of macrophage cultures.
9-氧代-10-吖啶乙酰氧肟酸（HCA）的钠盐是一种新的合成化合物，在水溶液中形成小晶体。这些晶体很容易被小鼠骨髓来源的巨噬细胞吞噬。摄入的HCA晶体在光学显微镜下可见为深色颗粒。吞噬程度通过对摄入的HCA吸收的光谱测量来估计。发现约10日龄的小鼠骨髓来源巨噬细胞培养物适合用于研究鼠源或人源干扰素的作用。观察到低浓度（10 - 200 U/ml）的鼠源α、β和γ干扰素刺激吞噬作用，而高浓度（400 - 1000 U/ml）则对吞噬作用无影响。用抗干扰素血清预先处理干扰素可消除其作用。发现CMA诱导的干扰素和生长因子可改变巨噬细胞培养物的吞噬活性。
