Song Bowen, Wei Wenkang, Liu Xueyi, Huang Yaoyao, Zhu Shuaiqi, Yi Lin, Ding Hongxing, Zhao Mingqiu, Chen Jinding
College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Agro-Biological Gene Research Center, State Key Laboratory of Livestock and Poultry Breeding, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
Vaccines (Basel). 2023 Oct 12;11(10):1587. doi: 10.3390/vaccines11101587.
Interferon (IFN) is a cell-secreted cytokine possessing biological activities including antiviral functioning, immune regulation, and others. Interferon-alpha (IFN-α) mainly derives from plasmacytoid dendritic cells, which activate natural killer cells and regulate immune responses. IFN-α responds to the primary antiviral mechanism in the innate immune system, which can effectively cure acute infectious diseases. Pseudorabies (PR) is an acute infectious disease caused by pseudorabies virus (PRV). The clinical symptoms of PRV are as follows: reproductive dysfunction among pregnant sows and high mortality rates among piglets. These pose a severe threat to the swine industry. Related studies show that IFN-α has broad applications in preventing and treating viral diseases. Therefore, a PRV mouse model using artificial infection was established in this study to explore the pathogenic effect of IFN-α on PRV. We designed a sequence with IFN-α4 (M28623, Genbank) and cloned it on the lentiviral vector. CHO-K1 cells were infected and identified using WB and RT-PCR; a CHO-K1 cell line with a stable expression of the recombinant protein PoIFN-α was successfully constructed. H&E staining and virus titer detection were used to investigate the recombinant protein PoIFN-α's effect on PR in BALB/c mice. The results show that the PoIFN-α has a preventive and therapeutic impact on PR. In conclusion, the recombinant protein can alleviate symptoms and reduce the replication of PRV in vivo.
干扰素（IFN）是一种细胞分泌的细胞因子，具有抗病毒、免疫调节等生物学活性。α干扰素（IFN-α）主要来源于浆细胞样树突状细胞，可激活自然杀伤细胞并调节免疫反应。IFN-α参与先天性免疫系统的主要抗病毒机制，能有效治愈急性传染病。伪狂犬病（PR）是由伪狂犬病病毒（PRV）引起的一种急性传染病。PRV的临床症状如下：妊娠母猪繁殖功能障碍以及仔猪高死亡率。这些给养猪业带来了严重威胁。相关研究表明，IFN-α在预防和治疗病毒性疾病方面具有广泛应用。因此，本研究建立了人工感染PRV的小鼠模型，以探讨IFN-α对PRV的致病作用。我们设计了一段含IFN-α4（M28623，Genbank）的序列，并将其克隆到慢病毒载体上。用WB和RT-PCR对感染的CHO-K1细胞进行鉴定；成功构建了稳定表达重组蛋白PoIFN-α的CHO-K1细胞系。采用苏木精-伊红（H&E）染色和病毒滴度检测来研究重组蛋白PoIFN-α对BALB/c小鼠伪狂犬病的作用。结果表明，PoIFN-α对伪狂犬病具有预防和治疗作用。总之，该重组蛋白可缓解症状并减少PRV在体内的复制。
