Waśniowska K, Duk M, Steuden I, Czerwiński M, Wiedłocha A, Lisowska E
Department of Immunochemistry, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.
Arch Immunol Ther Exp (Warsz). 1988;36(5):623-32.
Two monoclonal antibodies, N/61 (IgG2b) and N/92 (IgG2a), reacting preferentially with blood group N antigen, were obtained by immunization of BALB/c mice with human red cell membrane glycophorins. The antibodies showed distinctly different specificities. N/61 reacted with an epitope with NH2-terminal Leu residue, its free amino group, and sialic acid residues as essential components. The antibody N/92 reacted equally well with untreated and desialylated N glycoprotein, but did not react with de-O-glycosylated antigen. It seemed to recognize an epitope including both blood group N-characteristic amino acid residues, namely the 1st Leu and 5th Glu. The antibodies also differed in pH-dependence. N/61 showed maximal activity at pH 6-7 with tendency to decrease at higher pH values, whereas N/92 was not or weakly active up to pH 7, and showed a strong increase of activity at pH range 7-8.
通过用人红细胞膜糖蛋白免疫BALB/c小鼠，获得了两种单克隆抗体，N/61（IgG2b）和N/92（IgG2a），它们优先与血型N抗原发生反应。这些抗体表现出明显不同的特异性。N/61与一个表位发生反应，该表位以NH2末端的亮氨酸残基、其游离氨基和唾液酸残基作为必需成分。抗体N/92与未处理的和去唾液酸化的N糖蛋白反应同样良好，但不与去O-糖基化抗原反应。它似乎识别一个包括血型N特征性氨基酸残基（即第1个亮氨酸和第5个谷氨酸）的表位。这些抗体在pH依赖性方面也有所不同。N/61在pH 6 - 7时表现出最大活性，在较高pH值时活性有下降趋势，而N/92在pH 7之前无活性或活性较弱，在pH 7 - 8范围内活性强烈增加。
