Sonneborn H H, Ernst M
Dev Biol Stand. 1987;67:97-104.
The M/N-blood-group system is very suitable for investigating the reactivity of monoclonal antibodies because the antigenic structure seems to be very dependent on influences of the microenvironment, whereas on the other hand the biochemistry of these antigens is very well known. The distribution of these antigens within the population makes this system very interesting for forensic investigations and therefore one needs specific reagents with constant quality. We have immunized BALB/c mice with whole red blood cells and obtained a series of monoclonal antibodies. Some of these reacted with epitopes which are very dependent on pH, temperature and buffers concerning the binding of the antibodies and the specificity. By standardizing the monoclonal preparation in this respect it was possible to obtain specific reagents. In addition we found antibodies reacting with epitopes which were not influenced by the microenvironment. These antibodies always gave specific results. To get further criteria for standardization of these reagents we have adapted the method of flow cytometry for binding studies of monoclonal antibodies against red blood cell antigens.
M/N血型系统非常适合用于研究单克隆抗体的反应性，因为其抗原结构似乎非常依赖于微环境的影响，而另一方面，这些抗原的生物化学性质是广为人知的。这些抗原在人群中的分布使得该系统在法医调查中非常有趣，因此需要具有恒定质量的特定试剂。我们用全红细胞免疫BALB/c小鼠，获得了一系列单克隆抗体。其中一些与表位发生反应，这些表位在抗体结合和特异性方面非常依赖于pH、温度和缓冲液。通过在这方面对单克隆制剂进行标准化，有可能获得特定的试剂。此外，我们发现抗体与不受微环境影响的表位发生反应。这些抗体总是给出特定的结果。为了获得这些试剂标准化的进一步标准，我们采用了流式细胞术方法来研究抗红细胞抗原单克隆抗体的结合。
