Sonneborn H H, Uthemann H, Munro A C, Bruce M, Fraser R H, Inglis G
Dev Biol Stand. 1984;57:61-8.
In order to get more standardized reagents for blood group typing and to get more information on the chemical nature of blood group antigens we have immunized Balb/c mice with whole red blood cells and with sialoglycoprotein fraction from group OMMss cells. As fusion partners mouse myeloma cell lines P3-NS1/1-Ag 4-1 and X63-Ag8.653 were used. Several monoclonal antibodies were obtained, which showed specificity for the M/N antigens. Because of the relationship between M and N antigens this system is a very good example for studying the influence of different parameters on the reactivity of monoclonal antibodies. Each monoclonal antibody behaves like an individuum and for each antibody the dependence of the agglutination reaction of temperature, pH, buffer for dilution and medium, in which the reaction takes place, has to be investigated. Otherwise the results are not comparable and unspecific reactions might occur. By standardizing these parameters specific reactions were obtained and the antibodies might be used as routine reagents.
为了获得更标准化的血型分型试剂，并获取更多关于血型抗原化学性质的信息，我们用全红细胞和O型MMss细胞的唾液糖蛋白组分免疫了Balb/c小鼠。作为融合伙伴，使用了小鼠骨髓瘤细胞系P3-NS1/1-Ag 4-1和X63-Ag8.653。获得了几种单克隆抗体，它们对M/N抗原具有特异性。由于M和N抗原之间的关系，该系统是研究不同参数对单克隆抗体反应性影响的一个很好的例子。每种单克隆抗体的行为都像一个个体，对于每种抗体，都必须研究凝集反应对温度、pH值、稀释缓冲液以及反应发生的介质的依赖性。否则，结果将不可比，可能会发生非特异性反应。通过标准化这些参数，获得了特异性反应，这些抗体可用作常规试剂。
