Lints T, Holland R, Ralph R K
Department of Cellular and Molecular Biology, University of Auckland, New Zealand.
Biochim Biophys Acta. 1989 Oct 9;1013(3):287-93. doi: 10.1016/0167-4889(89)90148-1.
Arresting P815 mastocytoma cell growth with N6, O2'-dibutyryladenosine 3':5' cyclic monophosphate (db cAMP) and theophylline increased 45Ca2+ uptake and efflux by the cells (i.e, Ca2+ cycling) without altering cytoplasmic free Ca2+ concentrations or the amount or distribution of protein kinase C in the cells. Attempts to identify the Ca2+ channels involved using a wide variety of drugs were unsuccessful. However, the inhibitory effect of db cAMP on growth was greatly increase in medium containing low Ca2+ concentrations, confirming that interactions between Ca2+ and cyclic AMP can affect mastocytoma cell growth.
用N6, O2'-二丁酰腺苷3':5'-环磷酸（db cAMP）和茶碱抑制P815肥大细胞瘤细胞生长，可增加细胞对45Ca2+的摄取和外流（即Ca2+循环），而不会改变细胞内游离Ca2+浓度，也不会改变细胞中蛋白激酶C的量或分布。尝试使用多种药物来鉴定所涉及的Ca2+通道，但未成功。然而，在低Ca2+浓度的培养基中，db cAMP对生长的抑制作用大大增强，这证实了Ca2+与环磷酸腺苷之间的相互作用会影响肥大细胞瘤细胞的生长。
