Davis J, Ralph R K
Cancer Res. 1975 Jun;35(6):1495-504.
N6,O2'-Dibutyryladenosine cyclic 3',5'-phosphate plus theophylline inhibited the growth of the mouse mast cell tumor line PY 815 both in vivo and in vitro. The inhibitory effect on growth in vitro was rapidly reversed following removal of the drugs. Growth inhibition was accompanied by reduced cell surface activity and increased cell-cell adhesion. The drug-treated cells accumulated distinct membrane-bound granules, which are characteristic of more mature mast cells. Treated cells also developed increased amounts of surface-associated acidic mucopolysaccharides. These results suggest that increased intracellular cyclic adenosine 3':5'-monophosphate causes mouse mastocytoma cells to decrease growth and elicits the expression of a more differentiated mast cell phenotype. The effect of the antileukemia drug, 4'-(9-acridinylamino)methanesulfon-m-anisidine, on cyclic adenosine 3':5'-monophosphate and adenosine 5'-triphosphate in mastocytoma cells is also reported.
N6,O2'-二丁酰腺苷环3',5'-磷酸酯加茶碱在体内和体外均抑制小鼠肥大细胞瘤系PY 815的生长。去除药物后，体外生长抑制作用迅速逆转。生长抑制伴随着细胞表面活性降低和细胞间黏附增加。药物处理的细胞积累了独特的膜结合颗粒，这是更成熟肥大细胞的特征。处理后的细胞还产生了更多与表面相关的酸性黏多糖。这些结果表明，细胞内环腺苷3':5'-单磷酸增加会导致小鼠肥大细胞瘤细胞生长减少，并引发更分化的肥大细胞表型的表达。还报道了抗白血病药物4'-(9-吖啶基氨基)甲磺基间茴香胺对肥大细胞瘤细胞中环腺苷3':5'-单磷酸和三磷酸腺苷的影响。
