Alarifi Saud, Ali Daoud, Verma Ankit, Almajhdi Fahad N, Al-Qahtani Ahmed A
Department of Zoology, College of Science, King Saud University, Box 2454, 11451, Riyadh, Saudi Arabia.
In Vitro Cell Dev Biol Anim. 2014 Sep;50(8):714-22. doi: 10.1007/s11626-014-9760-3. Epub 2014 May 2.
Carbon nanotubes (CNTs) are gradually used in various areas including drug delivery, nanomedicine, biosensors, and electronics. The current study aimed to explore the DNA damage and cytotoxicity due to single-walled carbon nanotubes (SWCNTs) on human hepatocarcinoma cells (HepG2). Cellular proliferative assay showed the SWCNTs to exhibit a significant cell death in a dose- and time-dependent manner. However, SWCNTs induced significant intracellular reactive oxygen species (ROS) production and elevated lipid peroxidation, catalase, and superoxide dismutase in the HepG2 cells. SWCNTs also induced significant decrease in GSH and increase caspase-3 activity in HepG2 cells. DNA fragmentation analysis using the alkaline single-cell gel electrophoresis showed that the SWCNTs cause genotoxicity in a dose- and time-dependent manner. Therefore, the study points towards the capability of the SWCNTs to induce oxidative stress resulting cytotoxicity and genomic instability. This study warrants more careful assessment of SWCNTs before their industrial applications.
碳纳米管（CNTs）正逐渐应用于包括药物递送、纳米医学、生物传感器和电子学在内的各个领域。当前的研究旨在探索单壁碳纳米管（SWCNTs）对人肝癌细胞（HepG2）的DNA损伤和细胞毒性。细胞增殖试验表明，SWCNTs以剂量和时间依赖性方式导致显著的细胞死亡。然而，SWCNTs诱导HepG2细胞内活性氧（ROS）大量产生，并提高脂质过氧化、过氧化氢酶和超氧化物歧化酶水平。SWCNTs还导致HepG2细胞中谷胱甘肽显著减少，并增加半胱天冬酶-3活性。使用碱性单细胞凝胶电泳进行的DNA片段分析表明，SWCNTs以剂量和时间依赖性方式引起基因毒性。因此，该研究指出SWCNTs具有诱导氧化应激从而导致细胞毒性和基因组不稳定的能力。这项研究表明在SWCNTs进行工业应用之前需要进行更仔细的评估。
