• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

氧化应激导致氧化钴纳米颗粒诱导人肝癌细胞的细胞毒性和 DNA 损伤。

Oxidative stress contributes to cobalt oxide nanoparticles-induced cytotoxicity and DNA damage in human hepatocarcinoma cells.

机构信息

Cell and Molecular Laboratory, Department of Zoology, Faculty of Science, King Saud University, Riyadh, Saudi Arabia.

出版信息

Int J Nanomedicine. 2013;8:189-199. doi: 10.2147/IJN.S37924. Epub 2013 Jan 8.

DOI:10.2147/IJN.S37924
PMID:23326189
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3544340/
Abstract

BACKGROUND

Cobalt oxide nanoparticles (Co(3)O(4)NPs) are increasingly recognized for their utility in biological applications, magnetic resonance imaging, and drug delivery. However, little is known about the toxicity of Co(3)O(4)NPs in human cells.

METHODS

We investigated the possible mechanisms of genotoxicity induced by Co(3)O(4)NPs in human hepatocarcinoma (HepG2) cells. Cell viability, reactive oxygen species (ROS), glutathione, thiobarbituric acid reactive substance, apoptosis, and DNA damage were assessed in HepG2 cells after Co(3)O(4)NPs and Co(2+) exposure.

RESULTS

Co(3)O(4)NPs elicited a significant (P < 0.01) reduction in glutathione with a concomitant increase in lipid hydroperoxide, ROS generation, superoxide dismutase, and catalase activity after 24- and 48-hour exposure. Co(3)O(4)NPs had a mild cytotoxic effect in HepG2 cells; however, it induced ROS and oxidative stress, leading to DNA damage, a probable mechanism of genotoxicity. The comet assay showed a statistically significant (P < 0.01) dose- and time-related increase in DNA damage for Co(3)O(4)NPs, whereas Co(2+) induced less change than Co(3)O(4)NPs but significantly more than control.

CONCLUSION

Our results demonstrated that Co(3)O(4)NPs induced cytotoxicity and genotoxicity in HepG2 cells through ROS and oxidative stress.

摘要

背景

氧化钴纳米粒子（Co(3)O(4)NPs）因其在生物应用、磁共振成像和药物输送方面的应用而越来越受到关注。然而，人们对 Co(3)O(4)NPs 在人细胞中的毒性知之甚少。

方法

我们研究了 Co(3)O(4)NPs 在人肝癌（HepG2）细胞中诱导遗传毒性的可能机制。在 Co(3)O(4)NPs 和 Co(2+)暴露后，评估 HepG2 细胞中的细胞活力、活性氧（ROS）、谷胱甘肽、硫代巴比妥酸反应物质、细胞凋亡和 DNA 损伤。

结果

Co(3)O(4)NPs 在 24 小时和 48 小时暴露后，显著（P < 0.01）降低了谷胱甘肽，同时增加了脂质过氧化物、ROS 生成、超氧化物歧化酶和过氧化氢酶活性。Co(3)O(4)NPs 对 HepG2 细胞具有轻度细胞毒性作用；然而，它诱导了 ROS 和氧化应激，导致 DNA 损伤，这可能是遗传毒性的一种机制。彗星试验显示 Co(3)O(4)NPs 与 DNA 损伤呈剂量和时间相关的显著增加（P < 0.01），而 Co(2+) 引起的变化小于 Co(3)O(4)NPs，但明显大于对照。

结论

我们的结果表明，Co(3)O(4)NPs 通过 ROS 和氧化应激诱导 HepG2 细胞的细胞毒性和遗传毒性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a516/3544340/ea0aa77b7a75/ijn-8-189f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a516/3544340/741c99f90489/ijn-8-189f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a516/3544340/7e75c6997d1b/ijn-8-189f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a516/3544340/8c6338159adb/ijn-8-189f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a516/3544340/413f63537c1a/ijn-8-189f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a516/3544340/dae655815519/ijn-8-189f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a516/3544340/cd4bcef968f3/ijn-8-189f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a516/3544340/ea0aa77b7a75/ijn-8-189f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a516/3544340/741c99f90489/ijn-8-189f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a516/3544340/7e75c6997d1b/ijn-8-189f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a516/3544340/8c6338159adb/ijn-8-189f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a516/3544340/413f63537c1a/ijn-8-189f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a516/3544340/dae655815519/ijn-8-189f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a516/3544340/cd4bcef968f3/ijn-8-189f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a516/3544340/ea0aa77b7a75/ijn-8-189f7.jpg

相似文献

1
Oxidative stress contributes to cobalt oxide nanoparticles-induced cytotoxicity and DNA damage in human hepatocarcinoma cells.氧化应激导致氧化钴纳米颗粒诱导人肝癌细胞的细胞毒性和 DNA 损伤。
Int J Nanomedicine. 2013;8:189-199. doi: 10.2147/IJN.S37924. Epub 2013 Jan 8.
2
Cobalt iron oxide nanoparticles induce cytotoxicity and regulate the apoptotic genes through ROS in human liver cells (HepG2).氧化钴铁纳米颗粒在人肝细胞（HepG2）中通过活性氧诱导细胞毒性并调节凋亡基因。
Colloids Surf B Biointerfaces. 2016 Dec 1;148:665-673. doi: 10.1016/j.colsurfb.2016.09.047. Epub 2016 Sep 29.
3
Enhanced reactive oxygen species overexpression by CuO nanoparticles in poorly differentiated hepatocellular carcinoma cells.氧化铜纳米颗粒增强低分化肝癌细胞内活性氧的过度表达。
Nanoscale. 2015 Feb 7;7(5):1820-9. doi: 10.1039/c4nr05843g.
4
Quantitative Mapping of Oxidative Stress Response to Lithium Cobalt Oxide Nanoparticles in Single Cells Using Multiplexed in Situ Gene Expression Analysis.利用多重原位基因表达分析定量绘制单细胞中氧化应激反应对钴酸锂纳米颗粒的图谱。
Nano Lett. 2019 Mar 13;19(3):1990-1997. doi: 10.1021/acs.nanolett.8b05172. Epub 2019 Feb 21.
5
Comparative cytotoxicity and genotoxicity of cobalt (II, III) oxide, iron (III) oxide, silicon dioxide, and aluminum oxide nanoparticles on human lymphocytes in vitro.氧化钴（II、III）、氧化铁（III）、二氧化硅和氧化铝纳米颗粒对人淋巴细胞的体外比较细胞毒性和遗传毒性
Hum Exp Toxicol. 2016 Feb;35(2):170-83. doi: 10.1177/0960327115579208. Epub 2015 Mar 31.
6
Dose-dependent genotoxicity of copper oxide nanoparticles stimulated by reactive oxygen species in human lung epithelial cells.活性氧在人肺上皮细胞中刺激产生的氧化铜纳米颗粒的剂量依赖性遗传毒性。
Toxicol Ind Health. 2016 May;32(5):809-21. doi: 10.1177/0748233713511512. Epub 2013 Dec 5.
7
Molybdenum nanoparticles-induced cytotoxicity, oxidative stress, G2/M arrest, and DNA damage in mouse skin fibroblast cells (L929).钼纳米颗粒诱导的小鼠皮肤成纤维细胞（L929）的细胞毒性、氧化应激、G2/M 期阻滞和 DNA 损伤。
Colloids Surf B Biointerfaces. 2015 Jan 1;125:73-81. doi: 10.1016/j.colsurfb.2014.11.014. Epub 2014 Nov 20.
8
Copper oxide nanoparticles induced mitochondria mediated apoptosis in human hepatocarcinoma cells.氧化铜纳米颗粒诱导人肝癌细胞线粒体介导的细胞凋亡。
PLoS One. 2013 Aug 5;8(8):e69534. doi: 10.1371/journal.pone.0069534. Print 2013.
9
Enhanced Biocompatibility by Evaluating the Cytotoxic and Genotoxic Effects of Magnetic Iron Oxide Nanoparticles and Chitosan on Hepatocellular Carcinoma Cells (HCC).通过评估磁性氧化铁纳米粒子和壳聚糖对肝癌细胞（HCC）的细胞毒性和遗传毒性作用来提高生物相容性。
Cell Biochem Biophys. 2024 Jun;82(2):1027-1042. doi: 10.1007/s12013-024-01256-2. Epub 2024 Apr 1.
10
Genotoxic and oxidative stress effects of 2-amino-9H-pyrido[2,3-b]indole in human hepatoma G2 (HepG2) and human lung alveolar epithelial (A549) cells.2-氨基-9H-吡啶并[2,3-b]吲哚对人肝癌G2（HepG2）细胞和人肺泡上皮（A549）细胞的遗传毒性和氧化应激作用。
Toxicol Mech Methods. 2015 Mar;25(3):212-22. doi: 10.3109/15376516.2015.1025345. Epub 2015 Mar 23.

引用本文的文献

1
Cobalt oxide nanoparticles induce cytotoxicity and excessive ROS mediated mitochondrial dysfunction and p53-independent apoptosis in melanoma cells.氧化钴纳米颗粒在黑色素瘤细胞中诱导细胞毒性以及由过量活性氧介导的线粒体功能障碍和非p53依赖性凋亡。
Sci Rep. 2025 Jan 17;15(1):2220. doi: 10.1038/s41598-025-85691-y.
2
Ferroptosis resistance in cancer cells: nanoparticles for combination therapy as a solution.癌细胞中的铁死亡抗性：用于联合治疗的纳米颗粒作为一种解决方案。
Front Pharmacol. 2024 Jun 19;15:1416382. doi: 10.3389/fphar.2024.1416382. eCollection 2024.
3
Lithium and cobalt co-doped mesoporous bioactive glass nanoparticles promote osteogenesis and angiogenesis in bone regeneration.

本文引用的文献

1
Predictive toxicology of cobalt nanoparticles and ions: comparative in vitro study of different cellular models using methods of knowledge discovery from data.钴纳米粒子和离子的预测毒理学：使用数据知识发现方法对不同细胞模型的体外比较研究。
Toxicol Sci. 2011 Aug;122(2):489-501. doi: 10.1093/toxsci/kfr124. Epub 2011 May 20.
2
Olaquindox induces apoptosis through the mitochondrial pathway in HepG2 cells.奥拉喹多司通过线粒体途径诱导 HepG2 细胞凋亡。
Toxicology. 2011 Jul 29;285(3):104-13. doi: 10.1016/j.tox.2011.04.010. Epub 2011 Apr 27.
3
UVA-induced cyototoxicity and DNA damaging potential of benz (e) acephenanthrylene.
锂钴共掺杂介孔生物活性玻璃纳米粒子促进骨再生中的成骨作用和血管生成。
Front Bioeng Biotechnol. 2024 Jan 4;11:1288393. doi: 10.3389/fbioe.2023.1288393. eCollection 2023.
4
A New Cu/Fe Layer Double Hydroxide Nanocomposite Exerts Anticancer Effects against PC-3 Cells by Inducing Cell Cycle Arrest and Apoptosis.一种新型铜/铁层状双氢氧化物纳米复合材料通过诱导细胞周期阻滞和凋亡对PC-3细胞发挥抗癌作用。
Biomedicines. 2023 Aug 25;11(9):2386. doi: 10.3390/biomedicines11092386.
5
Evaluation of neurotoxicity and the role of oxidative stress of cobalt nanoparticles, titanium dioxide nanoparticles, and multiwall carbon nanotubes in Caenorhabditis elegans.评价神经毒性及钴纳米粒子、二氧化钛纳米粒子和多壁碳纳米管在秀丽隐杆线虫体内的氧化应激作用。
Toxicol Sci. 2023 Oct 30;196(1):85-98. doi: 10.1093/toxsci/kfad084.
6
Lignin-Cobalt Nano-Enabled Poly(pseudo)rotaxane Supramolecular Hydrogel for Treating Chronic Wounds.用于治疗慢性伤口的木质素-钴纳米增强聚（伪）轮烷超分子水凝胶
Pharmaceutics. 2023 Jun 13;15(6):1717. doi: 10.3390/pharmaceutics15061717.
7
Cobalt in athletes: hypoxia and doping - new crossroads.运动员体内的钴：低氧与兴奋剂——新的交叉点
J Appl Biomed. 2019 Mar;17(1):28. doi: 10.32725/jab.2018.003. Epub 2018 Nov 23.
8
Inspirations of Cobalt Oxide Nanoparticle Based Anticancer Therapeutics.基于氧化钴纳米颗粒的抗癌疗法的启示
Pharmaceutics. 2021 Oct 2;13(10):1599. doi: 10.3390/pharmaceutics13101599.
9
Combined Effects of Elevated Temperature and Crude Oil Pollution on Oxidative Stress and Apoptosis in Sea Cucumber (, Selenka).高温和原油污染对海参（，海地瓜）氧化应激和凋亡的联合影响。
Int J Environ Res Public Health. 2021 Jan 19;18(2):801. doi: 10.3390/ijerph18020801.
10
Cobalt oxide nanoparticles induce oxidative stress and alter electromechanical function in rat ventricular myocytes.钴氧化物纳米颗粒诱导大鼠心室肌细胞氧化应激并改变其机电功能。
Part Fibre Toxicol. 2021 Jan 6;18(1):1. doi: 10.1186/s12989-020-00396-6.
苯并（e）acephenanthrylene 的 UVA 诱导细胞毒性和 DNA 损伤潜力。
Toxicol Lett. 2010 Nov 30;199(2):193-200. doi: 10.1016/j.toxlet.2010.08.023. Epub 2010 Sep 9.
4
In vitro evaluation of SiC nanoparticles impact on A549 pulmonary cells: cyto-, genotoxicity and oxidative stress.体外评价碳化硅纳米颗粒对 A549 肺细胞的影响：细胞毒性、遗传毒性和氧化应激。
Toxicol Lett. 2010 Oct 20;198(3):324-30. doi: 10.1016/j.toxlet.2010.07.009. Epub 2010 Jul 23.
5
Silver nanospheres are cytotoxic and genotoxic to fish cells.银纳米球对鱼类细胞具有细胞毒性和遗传毒性。
Aquat Toxicol. 2010 Apr 1;97(1):34-41. doi: 10.1016/j.aquatox.2009.11.016. Epub 2009 Dec 2.
6
Evaluation of cell viability, DNA damage, and cell death in normal human dermal fibroblast cells induced by functionalized multiwalled carbon nanotube.功能化多壁碳纳米管诱导正常人类真皮成纤维细胞活力、DNA 损伤和细胞死亡的评价。
Mol Cell Biochem. 2010 May;338(1-2):225-32. doi: 10.1007/s11010-009-0356-2. Epub 2009 Dec 17.
7
Engineered cobalt oxide nanoparticles readily enter cells.工程化的氧化钴纳米颗粒很容易进入细胞。
Toxicol Lett. 2009 Sep 28;189(3):253-9. doi: 10.1016/j.toxlet.2009.06.851. Epub 2009 Jun 16.
8
Cell toxicity of superparamagnetic iron oxide nanoparticles.超顺磁性氧化铁纳米颗粒的细胞毒性
J Colloid Interface Sci. 2009 Aug 15;336(2):510-8. doi: 10.1016/j.jcis.2009.04.046. Epub 2009 Apr 22.
9
NanoGenotoxicology: the DNA damaging potential of engineered nanomaterials.纳米遗传毒理学：工程纳米材料的DNA损伤潜力
Biomaterials. 2009 Aug;30(23-24):3891-914. doi: 10.1016/j.biomaterials.2009.04.009. Epub 2009 May 8.
10
Cytotoxicity and genotoxicity of silver nanoparticles in human cells.银纳米颗粒对人体细胞的细胞毒性和遗传毒性。
ACS Nano. 2009 Feb 24;3(2):279-90. doi: 10.1021/nn800596w.