Li Le-Jun, Zhang Feng-Bin, Liu Shu-Yuan, Tian Yong-Hong, Le Fang, Wang Li-Ya, Lou Hang-Ying, Xu Xiang-Rong, Huang He-Feng, Jin Fan
Department of Reproductive Endocrinology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
Department of Reproductive Endocrinology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China Key Laboratory of Reproductive Genetics, National Ministry of Education (Zhejiang University), Women's Reproductive Health Laboratory of Zhejiang Province, Hangzhou, Zhejiang, China
Biol Reprod. 2014 Jun;90(6):125. doi: 10.1095/biolreprod.113.114827. Epub 2014 Apr 30.
In conventional in vitro fertilization (IVF), complete failure of fertilization occurs in 5% to 15% of treatments. Although the causes may be unclear, sperm defects appear to be the major contributor. However, a convincing test is not yet available that can predict the risk of fertilization failure. In this study, we found that germinal angiotensin-converting enzyme (gACE) (also called testicular ACE) was undetectable in sperm from patients who had total fertilization failure (TFF) and lower fertilization rates (LFRs) by IVF based on Western blot and indirect immunofluorescence analyses. Additionally, almost all of the patients without gACE on sperm (23 of 25) manifested a TT genotype of the rs4316 single-nucleotide polymorphism of ACE. Overall, our results indicate that the absence of gACE expression is responsible for TFF and LFRs by IVF. The rs4316 polymorphism of ACE might be associated with infertility in those patients. We conclude that sperm lacking gACE may be recognized before commencing IVF and that the patients may be directed instead to consider intracytoplasmic sperm injection.
在传统的体外受精（IVF）中，5%至15%的治疗会出现完全受精失败。尽管原因可能不明，但精子缺陷似乎是主要因素。然而，目前尚无令人信服的检测方法能够预测受精失败的风险。在本研究中，基于蛋白质印迹法和间接免疫荧光分析，我们发现，在体外受精时出现完全受精失败（TFF）和较低受精率（LFRs）的患者精子中，未检测到生殖型血管紧张素转换酶（gACE）（也称为睾丸ACE）。此外，精子上无gACE的几乎所有患者（25例中的23例）表现出ACE的rs4316单核苷酸多态性的TT基因型。总体而言，我们的结果表明，gACE表达缺失是体外受精导致TFF和LFRs的原因。ACE的rs4316多态性可能与这些患者的不孕有关。我们得出结论，在开始体外受精之前，可能识别出缺乏gACE的精子，并且这些患者可能会被建议考虑卵胞浆内单精子注射。
