Sahin Hüseyin, Akpak Yaşam Kemal, Berber Ufuk, Gün Ismet, Demirel Dilaver, Ergür Ali Rüştü
Department of Obstetrics and Gynaecology, Kasımpaşa Military Hospital, İstanbul, Turkey.
Department of Obstetrics and Gynaecology, Ankara Military Hospital, Ankara, Turkey.
J Turk Ger Gynecol Assoc. 2014 Mar 1;15(1):13-7. doi: 10.5152/jtgga.2014.56563. eCollection 2014.
Although trophoblastic invasion has a critical role in human placental development, very little is known about them. The aim of the present study was to localise the expression of P-cadherin (cadherin-3) and E-selectin in first trimester placenta.
This study was conducted on 140 patients who had applied to Gülhane Military Medical Academy, Haydarpaşa Education Hospital, Department of Obstetrics and Gynaecology between 2005 and 2006. The patients were divided into three groups: ectopic pregnancy group (Group 1), spontaneous abortion group (group 2) and curettage group (group 3 and/or control group). Patients with a history of systemic diseases (such as thrombophilia), a disease or anatomical diagnosis that may cause recurrent abortion or an aetiological factor for ectopic pregnancy were excluded from the study. Paraffin blocks were stained with E-selectin and P-cadherin in accordance with the procedure. Demographic characteristics of patients (patient age, gravida, parity, number of previous abortions, and last menstrual period) and staining intensities were compared using Analysis of Variance (ANOVA) among groups.
According to the average scale score of P-cadherin staining of cells, the three groups were statistically different from each other (p=0.0001). This difference stems from statistically significantly lower scores in the spontaneous abortion group than in both the ectopic pregnancy group (p<0.001) and the control group (p<0.001). E-selectin immunostaining showed no positive staining in the groups.
In placental trophoblasts, decreased P-cadherin immunoreactivity plays a role in the aetiopathogenesis of spontaneous abortion.
尽管滋养层细胞侵袭在人类胎盘发育中起关键作用，但人们对其了解甚少。本研究的目的是定位P-钙黏蛋白（钙黏蛋白-3）和E-选择素在孕早期胎盘的表达。
本研究对2005年至2006年间申请进入古勒汗军事医学院、海达尔帕夏教育医院妇产科的140例患者进行。患者分为三组：异位妊娠组（第1组）、自然流产组（第2组）和刮宫组（第3组和/或对照组）。有全身性疾病史（如血栓形成倾向）、可能导致反复流产的疾病或解剖学诊断或异位妊娠病因的患者被排除在研究之外。石蜡块按照程序用E-选择素和P-钙黏蛋白染色。使用方差分析（ANOVA）比较各组患者的人口统计学特征（患者年龄、孕次、产次、既往流产次数和末次月经）和染色强度。
根据细胞P-钙黏蛋白染色的平均量表评分，三组之间在统计学上存在差异（p = 0.0001）。这种差异源于自然流产组的评分在统计学上显著低于异位妊娠组（p < 0.001）和对照组（p < 0.001）。E-选择素免疫染色在各组中均未显示阳性染色。
在胎盘滋养层细胞中，P-钙黏蛋白免疫反应性降低在自然流产的发病机制中起作用。
