Department of Microbiology, Faculty of biology, State University of Baku, Azarbayjan.
Bioimpacts. 2014;4(1):49-52. doi: 10.5681/bi.2014.014. Epub 2014 Apr 7.
Lactobacillus is believed to be beneficial in human health, thus the search for isolation and identification of friendly human bacteria from traditional fermented foods is important in medicine. One of the dairy products, traditional cheese as a highly-consumed dairy product could be a valuable source of these friendly edible germs.
In this research, home-made cheese from Lankaran, Jalil Abad and Salian regions in Azerbaijan was characterized for the presence of Lactobacilli with probiotic potential. The bacterial suspension was enriched and screened for acid and bile resistances. Then, the isolates were subjected to antibiotic resistance and antibacterial effects against convenient pathogenic bacteria. The isolates were identified by 16s rDNA sequencing.
The results clearly revealed two probiotics with higher homology to Lactobacillus planetarum and Lactobacillus fermentum.
No antibiotic resistance was detected in any of the potentially probiotic lactobacilli isolates in these regions, where people continue to follow a life-style that is largely traditional, with traditional medications.
人们认为乳杆菌对人类健康有益，因此从传统发酵食品中分离和鉴定对人体友好的细菌在医学上很重要。乳制品之一，传统奶酪作为一种高消费的乳制品，可能是这些可食用友好细菌的有价值来源。
在这项研究中，对来自阿塞拜疆的兰卡尔、贾利勒阿巴德和萨利安地区的自制奶酪进行了研究，以确定具有益生菌潜力的乳杆菌。细菌悬浮液经过富集和筛选，以抵抗酸和胆汁。然后，将分离株进行抗生素耐药性和对抗方便致病菌的抗菌作用测试。通过 16s rDNA 测序对分离株进行鉴定。
结果清楚地显示了两种与植物乳杆菌和发酵乳杆菌具有更高同源性的益生菌。
在这些地区，人们继续遵循传统的生活方式，使用传统药物，没有发现任何潜在益生菌乳杆菌分离株具有抗生素耐药性。
