Department of Food and Biotechnology, Woosuk University, Jeonju, Republic of Korea.
J Microbiol. 2010 Aug;48(4):467-72. doi: 10.1007/s12275-010-0204-9. Epub 2010 Aug 20.
Two lactic acid bacteria (LAB) having ornithine-producing capacity were isolated from Korean natural sea salt. They were Gram-positive, short rod-type bacteria, and able to grow anaerobically with CO(2) production. The isolates grew well on MRS broth at 30-37 degrees C and a pH of 6.5-8.0. The optimum temperature and pH for growth are 37 degrees C and pH 7.0. The isolates fermented D-ribose, D-galactose, D-lactose, D-maltose, Dcellobiose, D-tagatose, D-trehalose, sucrose, D-melezitose, gentiobiose, D-glucose but not D-melibiose, inositol, and L-sorbose. The 16S rDNA sequences of the two isolates showed 99.5% and 99.6% homology with the Weissella koreensis S5623 16S rDNA (Access no. AY035891). They were accordingly identified and named as Weissella koreensis MS1-3 and Weissella koreensis MS1-14, and produced intracellular ornithine at levels of 72 mg/100 g cell F.W. and 105 mg/100 g cell F.W. and extracellular ornithine at levels of 4.5 mg/100 ml and 4.6 mg/100 ml medium, respectively, by culturing in MRS broth supplemented with 1% arginine. High cell growth was maintained in MRS broth with a NaCl concentration of 0-6%. These results show for the first time that Korean natural sea salts contain lactic acid bacteria Weissella koreensis strains having ornithine producing capacity.
从韩国天然海盐中分离到两株具有产鸟氨酸能力的乳酸菌（LAB）。它们是革兰氏阳性、短杆状细菌，能够在产生 CO(2)的情况下进行厌氧生长。这些分离物在 MRS 肉汤中 30-37°C 和 pH6.5-8.0 下生长良好。最佳生长温度和 pH 值分别为 37°C 和 pH7.0。这些分离物发酵 D-核糖、D-半乳糖、D-乳糖、D-麦芽糖、D-纤维二糖、D-塔格糖、D-海藻糖、蔗糖、D-密二糖、龙胆二糖、D-葡萄糖，但不发酵 D-棉子糖、肌醇和 L-山梨糖。这两个分离株的 16S rDNA 序列与 Weissella koreensis S5623 16S rDNA（登录号 AY035891）的同源性分别为 99.5%和 99.6%。因此，它们被鉴定并命名为 Weissella koreensis MS1-3 和 Weissella koreensis MS1-14，在 MRS 肉汤中培养时，细胞干重的胞内鸟氨酸产量分别为 72mg/100g 和 105mg/100g，细胞外鸟氨酸产量分别为 4.5mg/100ml 和 4.6mg/100ml 培养基。在补充 1%精氨酸的 MRS 肉汤中培养时，细胞生长良好，在 0-6%NaCl 浓度的 MRS 肉汤中也能维持高细胞生长。这些结果首次表明，韩国天然海盐中含有具有产鸟氨酸能力的乳酸菌 Weissella koreensis 菌株。
