Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Food Science and Technology, Faculty of Agriculture Science, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
Food Chem. 2019 Jan 30;272:709-714. doi: 10.1016/j.foodchem.2018.08.081. Epub 2018 Aug 20.
Lactobacillus species are beneficial for the functional food industry and preventive medicine. The complex microflora of traditional cheese depends on the cheese types (e.g., homemade rennets). Here, the abomasum driven rennet was assessed for the existence of lactobacilli. For differentiating lactobacilli, the bacterial suspension was screened for the acid and bile resistance. The isolated bacteria were evaluated for antibiotic susceptibility and antagonistic impacts on other pathogenic bacteria. The 16S rDNA gene was evaluated by the amplified ribosomal DNA restriction analysis (ARDRA) recruiting the restriction enzyme Taq I and compared to the virtually digested patterns of previous reports on lactobacilli. The isolates were examined by random amplified polymorphic DNA (RAPD) and distinctive lactobacilli were sequenced. ARDRA and RAPD data showed three distinct lactobacilli strains, including L. acidophilus, L. planetarum, and L. fermentum. The homemade rennet is proposed as the novel source of probiotic strains as an alternative to the traditional cheeses.
乳杆菌属对功能性食品工业和预防医学有益。传统奶酪的复杂微生物群依赖于奶酪类型（例如，自制凝乳酶）。在这里，评估了皱胃驱动的凝乳酶中是否存在乳杆菌。为了区分乳杆菌，对细菌悬浮液进行了酸和胆汁抗性筛选。对分离出的细菌进行了抗生素敏感性评估，并评估了其对其他病原菌的拮抗作用。通过扩增核糖体 DNA 限制分析 (ARDRA) 评估 16S rDNA 基因，该方法使用限制酶 Taq I，并与先前关于乳杆菌的虚拟消化模式进行比较。通过随机扩增多态性 DNA (RAPD) 对分离株进行检查，并对独特的乳杆菌进行测序。ARDRA 和 RAPD 数据显示了三种不同的乳杆菌菌株，包括嗜酸乳杆菌、植物乳杆菌和发酵乳杆菌。建议将自制凝乳酶作为益生菌菌株的新来源，以替代传统奶酪。
