School of Biotechnology, University of Jammu, Bawe Wali Rakh, Jammu, 180006, India.
Probiotics Antimicrob Proteins. 2016 Sep;8(3):121-9. doi: 10.1007/s12602-016-9220-3.
Wide variations among multifaceted-health benefitting attributes of probiotics fueled investigations on targeting efficacious probiotics. In the current study, lactic acid bacteria (LAB) isolated from poultry gut, feces of rat, chicken, human infants, and fermented foods were characterized for desired probiotic functional properties including the phytase-producing ability which is one of the wanted characteristics for probiotics for potential applications for upgrading animal nutrition, enhancing feed conversion, and minimizing anti-nutritional properties. Among 62 LAB isolates Weissella kimchii R-3 an isolate from poultry gut exhibited substantial phytase-producing ability (1.77 U/ml) in addition to other functional probiotic characteristics viz. hydrophobicity, autoaggregation, coaggregation with bacterial pathogens, and antimicrobial activity against pathogens. Survival of W. kimchii R-3 cells (in free and calcium alginate encapsulated state) was examined sequentially in simulated gastric and intestinal juices. Encapsulated cells exhibited better survival under simulated gut conditions indicating that encapsulation conferred considerable protection against adverse gut conditions. Furthermore, simulated gastric and intestinal juices with pepsin and pancreatin showed higher survival of cells than the juices without pepsin and pancreatin. W. kimchii R-3 due to its significant functional probiotic attributes may have prospective for commercial applications in human/animal nutrition.
益生菌具有多种有益于健康的特性，差异很大，这促使人们对靶向有效益生菌进行了研究。在本研究中，从禽类肠道、大鼠粪便、鸡、人类婴儿和发酵食品中分离出的乳酸菌（LAB），其特性是具有所需的益生菌功能特性，包括植酸酶产生能力，这是益生菌的一个理想特性，可潜在应用于改善动物营养、提高饲料转化率和最小化抗营养特性。在 62 株 LAB 分离株中，从禽类肠道分离出的魏斯氏菌（Weissella kimchii）R-3 菌株除了具有其他功能性益生菌特性（如疏水性、自动聚集、与细菌病原体的共聚以及对病原体的抗菌活性）外，还具有很强的植酸酶产生能力（1.77 U/ml）。随后在模拟胃液和肠液中检测了 W. kimchii R-3 细胞（游离和海藻酸钙包埋状态）的存活情况。包埋细胞在模拟肠道条件下表现出更好的存活能力，表明包埋赋予了细胞对不利肠道条件的相当大的保护。此外，含有胃蛋白酶和胰蛋白酶的模拟胃液和肠液比不含胃蛋白酶和胰蛋白酶的消化液显示出更高的细胞存活率。由于其显著的功能性益生菌特性，W. kimchii R-3 可能具有在人类/动物营养方面的商业应用前景。
