Division of Cell Biology, Chemical Biology and Drug Innovation, The Netherlands Cancer Institute Amsterdam, Netherlands.
Front Chem. 2014 Apr 1;2:15. doi: 10.3389/fchem.2014.00015. eCollection 2014.
Unnatural amino acid (UAA) incorporation by amber codon suppression offers scientists a powerful tool to modify the properties of proteins at will. UAA incorporation has been used for a plethora of fundamental research applications and, more recently, also for the selective modification of therapeutic proteins. In this review most recent developments in Escherichia coli codon expansion and, unnatural amino acid incorporation are discussed together with some remarkable recent developments in improved efficient UAA incorporation. We focus on the generation of proteins that hold promise for future therapeutic applications that would be impossible to obtain without unnatural amino acid incorporation, including the generation of bi-specific antibodies and antibody drug conjugates.
非天然氨基酸（UAA）通过琥珀终止密码子抑制的掺入为科学家提供了一种强大的工具，可随心所欲地修饰蛋白质的性质。UAA 的掺入已被广泛用于各种基础研究应用，最近也用于治疗性蛋白质的选择性修饰。在这篇综述中，讨论了大肠杆菌密码子扩展和非天然氨基酸掺入的最新进展，以及在提高 UAA 掺入效率方面的一些显著进展。我们专注于生成具有未来治疗应用前景的蛋白质，如果没有非天然氨基酸掺入，这些蛋白质是不可能获得的，包括生成双特异性抗体和抗体药物偶联物。
