[研究GK-2（h）——一种新型人神经生长因子原始模拟物的体外神经保护特性]

[Studying in vitro neuroprotective properties of GK-2(h)--a new original mimetic of human nerve growth factor].

作者信息

Antipova T A, Nikolaev S V, Gudasheva T A, Seredenin S B

出版信息

Eksp Klin Farmakol. 2014;77(2):8-11.

Abstract

Human nerve growth factor (NGF) mimetic hexamethylenediamide bis-N-monosuccinyl-glicyl-lysine) (GK-2(h)) demonstrates protective activity on immortalized mouse hippocampal cell line HT22 in a concentration up to 10(-13)M against hydrogen peroxide toxicity and in concentration up to 10(-8) M against glutamate toxicity. GK-2(h) dipeptide protects rat PC-12 pheochromocytoma cells in a concentration up to 10(-7) M and protects human neuroblastoma SH-SY5Y cells in a concentration up to 10(-8) against 6-hydroxydofamine-induced damage.

摘要

人神经生长因子（NGF）模拟物六亚甲基二胺双-N-单琥珀酰-甘氨酰-赖氨酸（GK-2(h)）在浓度高达10^(-13)M时，对永生化小鼠海马细胞系HT22具有抗过氧化氢毒性的保护活性，在浓度高达10^(-8)M时，具有抗谷氨酸毒性的保护活性。GK-2(h)二肽在浓度高达10^(-7)M时可保护大鼠嗜铬细胞瘤PC-12细胞，在浓度高达10^(-8)时可保护人神经母细胞瘤SH-SY5Y细胞免受6-羟基多巴胺诱导的损伤。