评价链脲佐菌素-烟酰胺诱导的糖尿病大鼠的代谢和免疫变化。
Evaluation of metabolic and immunological changes in streptozotocin-nicotinamide induced diabetic rats.
机构信息
Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Malaysia.
Immunology Unit, Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Malaysia.
出版信息
Cell Immunol. 2014 May-Jun;289(1-2):145-9. doi: 10.1016/j.cellimm.2014.04.004. Epub 2014 Apr 18.
Type 2 diabetes is a chronic disease with growing public health concern globally. Finding remedies to assist this health issue requires recruiting appropriate animal model for experimental studies. This study was designated to evaluate metabolic and immunologic changes in streptozotocin-nicotinamide induced diabetic rats as a model of type 2 diabetes. Male rats were induced diabetes using nicotinamide (110 mg/kg) and streptozotocin (65 mg/kg). Following 42 days, biochemical and immunological tests showed that diabetic rats had higher levels of blood glucose, WBC, certain abnormalities in lipid profile and insufficient mitogenic responses of lymphocytes (p<0.05). However, the status of the total antioxidant, inflammatory biomarkers and other parameters of full blood count (except HCT) were not significantly altered. Phenotyping assay indicated insignificant lymphocyte subtype imbalances excluding a significant rise in the level of CD4+CD25+ marker (p<0.05). This model of diabetic animals may represent some but not all symptoms of human type 2 diabetes.
2 型糖尿病是一种全球性的慢性疾病，越来越受到公众健康的关注。寻找治疗方法来帮助解决这个健康问题，需要招募合适的动物模型进行实验研究。本研究旨在评估链脲佐菌素-烟酰胺诱导的糖尿病大鼠模型的代谢和免疫变化。雄性大鼠用烟酰胺（110mg/kg）和链脲佐菌素（65mg/kg）诱导糖尿病。42 天后，生化和免疫测试显示，糖尿病大鼠的血糖、白细胞（WBC）水平更高，血脂谱存在某些异常，淋巴细胞的有丝分裂反应不足（p<0.05）。然而，总抗氧化剂、炎症生物标志物和全血细胞计数（除 HCT 外）的其他参数的状态没有明显改变。表型分析表明，除 CD4+CD25+标志物水平显著升高外（p<0.05），淋巴细胞亚型失衡并不显著。这种糖尿病动物模型可能代表了人类 2 型糖尿病的一些症状，但不是全部。
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