Kunert J
Acta Univ Palacki Olomuc Fac Med. 1989;122:25-38.
Twenty-six keratinolytic fungi (16 dermatophytes and 10 soil species) and sixteen non-keratinolytic fungi were cultivated on sterile human hair in a mineral solution. With keratinolytic fungi, the loss in total dry weight (hair + mycelium) reached 7.6 up to 24.2% after 60 days of cultivation. Peptidic (Lowry-positive) substances accumulated in the medium and pH of the medium gradually increased, often over pH 8. Excess of sulfur contained in the substrate was excreted back into the medium predominantly in the form of inorganic sulfate. Moreover, sulfite was produced that cleaved disulfide bonds of the substrate giving rise to S-sulfocysteine; soluble products of keratin degradation in the medium contained 2.1 to 6.5% of that substance. This supports the presumption that sulfitolysis of disulfide bonds is a key reaction of keratinolysis in fungi. Statistically significant correlations were observed between substrate degradation and medium alkalinization, as well as the contents of peptidic substances, sulfate, and S-sulfocysteine. The correlation was highest with sulfate content and least significant with peptidic substances. Non-keratinolytic fungi mostly grew on the hair but did not cause a gravimetrically measurable loss of the substrate, did not alkalinize the medium, and did not accumulate the above mentioned substances in amounts comparable with those of keratinolytic species.
将26种角蛋白分解真菌（16种皮肤癣菌和10种土壤真菌）和16种非角蛋白分解真菌在无菌人发上于矿物溶液中培养。对于角蛋白分解真菌，培养60天后，总干重（毛发+菌丝体）损失达7.6%至24.2%。培养基中积累了肽类（Lowry阳性）物质，培养基的pH值逐渐升高，常超过pH 8。底物中所含的过量硫主要以无机硫酸盐的形式排泄回培养基中。此外，还产生了亚硫酸盐，其裂解底物的二硫键，生成S-磺基半胱氨酸；培养基中角蛋白降解的可溶性产物含有该物质的2.1%至6.5%。这支持了二硫键的亚硫酸解是真菌角蛋白分解的关键反应这一推测。在底物降解与培养基碱化以及肽类物质、硫酸盐和S-磺基半胱氨酸含量之间观察到具有统计学意义的相关性。与硫酸盐含量的相关性最高，与肽类物质的相关性最不显著。非角蛋白分解真菌大多在毛发上生长，但未导致底物出现重量可测的损失，未使培养基碱化，也未积累与角蛋白分解真菌数量相当的上述物质。
