Kaul S, Sumbali G
Department of Biosciences, University of Jammu, India.
Mycopathologia. 1997;139(3):137-40. doi: 10.1023/a:1006896030739.
Species of Chrysosporium, Malbranchea, Scopulariopsis, Microascus, and Gliocladium isolated from poultry farm soils were grown on two keratin media. All the test fungi could grow on keratin (human hair) and degrade it, releasing sulphydryl-containing compounds detected as extracellular keratinase, cysteine and total proteins. Glucose supplemented keratin medium supported maximum keratinolysis. Keratinase activity also changed the pH of the medium towards alkalinity and species with strong keratinolytic ability were noted to render the culture medium more alkaline than those that were less kiratinolytic.
从家禽养殖场土壤中分离出的金孢子菌属、马尔布兰奇霉属、帚霉属、小囊菌属和黏帚霉属的菌种,在两种角蛋白培养基上生长。所有受试真菌都能在角蛋白(人发)上生长并将其降解,释放出含巯基的化合物,这些化合物被检测为细胞外角蛋白酶、半胱氨酸和总蛋白。添加葡萄糖的角蛋白培养基支持最大程度的角蛋白分解。角蛋白酶活性还使培养基的pH值向碱性变化,并且发现具有较强角蛋白分解能力的菌种比角蛋白分解能力较弱的菌种使培养基的碱性更强。