Zhang Jingyao, Wu Qifei, Song Sidong, Wan Yong, Zhang Ruiyao, Tai Minghui, Liu Chang
Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
Department of Thoracic Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
Int Immunopharmacol. 2014 Jul;21(1):94-101. doi: 10.1016/j.intimp.2014.04.011. Epub 2014 Apr 29.
To study the effect of hydrogen-rich water (HRW) on acute peritonitis with three different rat models.
Acute peritonitis was induced by three methods including intraperitoneal injection of lipopolysaccharide (LPS), rats' feces or cecal ligation and puncture (CLP) operation. For each model, male Sprague Dawley rats were used and distributed into saline control group, HRW control group, saline plus model group, and HRW plus model group. Saline or HRW (3 ml per rat) was orally administered by gavage for 7 days beforehand and 3 days after modeling. The efficacy was tested by detecting concentrations of white blood cells (WBCs), plasma endotoxin, interleukin (IL)-6 and tumor necrosis factor (TNF)-α. The activities of malondialdehyde (MDA), myeloperoxidase (MPO) and glutathione (GSH) in visceral peritoneum tissues were also evaluated. Meanwhile, histopathology examination of visceral peritoneum was performed using hematoxylin and eosin staining. The expression and location of nuclear factor kappaB (NF-κB) in the visceral peritoneum were detected by immunohistochemistry.
Three models showed the same result that hydrogen-rich water had an efficient protective effect on acute peritonitis. HRW could significantly lower the levels of WBCs, plasma endotoxin and cytokines, enhance GSH activity and reduce MPO and MDA activities in the peritoneum tissue when compared with that of groups with only saline treated. Simultaneously, we found that HRW could also decrease the NF-κB expression in the peritoneum tissues.
Hydrogen-rich water could alleviate the severity of acute peritonitis, and it might perform this function by its anti-inflammation, anti-oxidation and anti-bacterial effects and reducing NF-κB expression in the peritoneum tissues.
采用三种不同的大鼠模型研究富氢水（HRW）对急性腹膜炎的影响。
通过三种方法诱导急性腹膜炎，包括腹腔注射脂多糖（LPS）、大鼠粪便或盲肠结扎穿刺（CLP）手术。对于每种模型，使用雄性Sprague Dawley大鼠，并将其分为生理盐水对照组、HRW对照组、生理盐水加模型组和HRW加模型组。在建模前7天和建模后3天，通过灌胃法给大鼠口服生理盐水或HRW（每只大鼠3 ml）。通过检测白细胞（WBC）、血浆内毒素、白细胞介素（IL）-6和肿瘤坏死因子（TNF）-α的浓度来测试疗效。还评估了内脏腹膜组织中丙二醛（MDA）、髓过氧化物酶（MPO）和谷胱甘肽（GSH）的活性。同时，采用苏木精和伊红染色对内脏腹膜进行组织病理学检查。通过免疫组织化学检测核因子κB（NF-κB）在内脏腹膜中的表达和定位。
三种模型显示出相同的结果，即富氢水对急性腹膜炎具有有效的保护作用。与仅用生理盐水处理的组相比，HRW可显著降低腹膜组织中WBC、血浆内毒素和细胞因子的水平，增强GSH活性，降低MPO和MDA活性。同时，我们发现HRW还可降低腹膜组织中NF-κB的表达。
富氢水可减轻急性腹膜炎的严重程度，其可能通过抗炎、抗氧化和抗菌作用以及降低腹膜组织中NF-κB的表达来发挥这一功能。
