狂犬病病毒糖蛋白假型重组杆状病毒疫苗在小鼠模型中可完全预防致命狂犬病病毒攻击。

Rabies-virus-glycoprotein-pseudotyped recombinant baculovirus vaccine confers complete protection against lethal rabies virus challenge in a mouse model.

机构信息

Division of Animal Infectious Diseases, State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.

Division of Animal Infectious Diseases, State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; Departments of Pathology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.

出版信息

Vet Microbiol. 2014 Jun 25;171(1-2):93-101. doi: 10.1016/j.vetmic.2014.03.037. Epub 2014 Apr 3.

DOI:10.1016/j.vetmic.2014.03.037

PMID:24793501

Abstract

Rabies virus has been an ongoing threat to humans and animals. Here, we developed a new strategy to generate a rabies virus vaccine based on a pseudotyped baculovirus. The recombinant baculovirus (BV-RVG/RVG) was pseudotyped with the rabies virus glycoprotein (RVG) and also simultaneously expressed another RVG under the control of the immediate early CMV promoter. In vitro, this RVG-pseudotyped baculovirus vector induced syncytium formation in insect cells and displayed more efficient gene delivery into mammalian cells. Mice immunized with BV-RVG/RVG developed higher levels of virus-neutralizing antibodies, and conferred 100% protection against rabies viral challenge. These data indicate that the RVG-pseudotyped baculovirus BV-RVG/RVG can be used as an alternative strategy to develop a safe and efficacious vaccine against the rabies virus.

摘要

狂犬病病毒一直是人类和动物的持续威胁。在这里，我们开发了一种新的策略，基于假型杆状病毒来生产狂犬病病毒疫苗。重组杆状病毒（BV-RVG/RVG）被狂犬病病毒糖蛋白（RVG）假型化，同时在即刻早期 CMV 启动子的控制下表达另一个 RVG。在体外，这种 RVG 假型化杆状病毒载体诱导昆虫细胞中的合胞体形成，并显示出更有效的基因递送至哺乳动物细胞。用 BV-RVG/RVG 免疫的小鼠产生了更高水平的病毒中和抗体，并对狂犬病病毒攻击提供了 100%的保护。这些数据表明，RVG 假型化杆状病毒 BV-RVG/RVG 可用作开发针对狂犬病病毒的安全有效疫苗的替代策略。