National Facility for High Field NMR, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400 005, India.
Principal K.M. Kundnani College of Pharmacy, Cuffe Parade, Mumbai 400 005, India.
Eur J Med Chem. 2014 Jun 10;80:285-94. doi: 10.1016/j.ejmech.2014.04.054. Epub 2014 Apr 22.
The localization and interaction of six naturally occurring flavones (FLV, 5HF, 6HF, 7HF, CHY and BLN) in DPPC bilayers were studied using DSC and multi-nuclear NMR. DSC results indicate that FLV and 6HF interact with alkyl chains. The (1)H NMR shows interaction of flavones with the sn-glycero region. Ring current induced chemical shifts indicate that 6HF and BLN acquire parallel orientation in bilayers. 2D NOESY spectra indicate partitioning of the B-ring into the alkyl chain region. The DSC, NMR and binding studies indicate that 5HF and 7HF are located near head group region, while 6HF, CHY and BLN are located in the vicinity of sn-glycero region, and FLV is inserted deepest in the membrane.
使用 DSC 和多核 NMR 研究了六种天然类黄酮（FLV、5HF、6HF、7HF、CHY 和 BLN）在 DPPC 双层中的定位和相互作用。DSC 结果表明 FLV 和 6HF 与烷基链相互作用。(1)H NMR 显示类黄酮与 sn-甘油区域相互作用。环流诱导的化学位移表明 6HF 和 BLN 在双层中获得平行取向。2D NOESY 谱表明 B 环部分分配到烷基链区域。DSC、NMR 和结合研究表明，5HF 和 7HF 位于头部基团区域附近，而 6HF、CHY 和 BLN 位于 sn-甘油区域附近，FLV 则最深插入膜中。
