Dos Santos Desirée Magalhães, Rocha Camila Valesca Jardim, da Silveira Elita Ferreira, Marinho Marcelo Augusto Germani, Rodrigues Marisa Raquel, Silva Nichole Osti, da Silva Ferreira Ailton, de Moura Neusa Fernandes, Darelli Gabriel Jorge Sagrera, Braganhol Elizandra, Horn Ana Paula, de Lima Vânia Rodrigues
Programa de Pós-Graduação em Química Tecnológica e Ambiental, Escola de Química e Alimentos, Universidade Federal do Rio Grande, Av. Itália, km 8, Campus Carreiros, Rio Grande, RS, 96203-900, Brazil.
Programa de Pós Graduação em Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Av. Itália, km 8, Campus Carreiros, Rio Grande, RS, 96203-900, Brazil.
J Membr Biol. 2018 Apr;251(2):247-261. doi: 10.1007/s00232-018-0017-z. Epub 2018 Feb 8.
Rapanea ferruginea antioxidant and antitumoral properties were not explored before in literature. This study aimed to investigate these biological activities for the R. ferruginea leaf extract and correlate them with its phenolic content and influence in biological membrane dynamics. Thus, in this study, anti/pro-oxidative properties of R. ferruginea leaf extract by in vitro DPPH and TBARS assays, with respect to the free radical reducing potential and to its activity regarding membrane free radical-induced peroxidation, respectively. Furthermore, preliminary tests related to the extract effect on in vitro glioma cell viability were also performed. In parallel, the phenolic content was detected by HPLC-DAD and included syringic and trans-cinnamic acids, quercetrin, catechin, quercetin, and gallic acid. In an attempt to correlate the biological activity of R. ferruginea extract and its effect on membrane dynamics, the molecular interaction between the extract and a liposomal model with natural-sourced phospholipids was investigated. Location and changes in vibrational, rotational, and translational lipid motions, as well as in the phase state of liposomes, induced by R. ferruginea extract, were monitored by Fourier-transform infrared spectroscopy, nuclear magnetic resonance, differential scanning calorimetry, and UV-visible spectroscopy. In its free form, the extract showed promising in vitro antioxidant properties. Free-form extract (at 1000µ g/mL) exposure reduced glioma cell in vitro viability in 40%, as evidenced by MTT tests. Pro-oxidant behavior was observed when the extract was loaded into liposomes. A 70.8% cell viability reduction was achieved with 500 µg/mL of liposome-loaded extract. The compounds of R. ferruginea extract ordered liposome interface and disorder edits a polar region. Phenolic content, as well as membrane interaction and modulation may have an important role in the oxidative and antitumoral activities of the R. ferruginea leaf extract.
文献中此前尚未对铁锈树（Rapanea ferruginea）的抗氧化和抗肿瘤特性进行研究。本研究旨在探究铁锈树叶提取物的这些生物活性，并将其与酚类含量以及对生物膜动力学的影响相关联。因此，在本研究中，通过体外DPPH和TBARS测定法分别研究了铁锈树叶提取物的抗氧化/促氧化特性，即其自由基还原潜力以及对膜自由基诱导的过氧化反应的活性。此外，还进行了与提取物对体外胶质瘤细胞活力影响相关的初步测试。同时，通过HPLC-DAD检测酚类含量，其中包括丁香酸、反式肉桂酸、槲皮苷、儿茶素、槲皮素和没食子酸。为了将铁锈树提取物的生物活性与其对膜动力学的影响相关联，研究了该提取物与具有天然来源磷脂的脂质体模型之间的分子相互作用。通过傅里叶变换红外光谱、核磁共振、差示扫描量热法和紫外可见光谱监测了铁锈树提取物诱导的脂质体振动、旋转和平动运动以及脂质体相态的位置和变化。提取物以游离形式显示出有前景的体外抗氧化特性。MTT试验证明，1000μg/mL的游离形式提取物可使胶质瘤细胞的体外活力降低40%。当提取物负载到脂质体中时，观察到促氧化行为。500μg/mL的脂质体负载提取物可使细胞活力降低70.8%。铁锈树提取物的化合物使脂质体界面有序化，并使极性区域无序化。酚类含量以及膜相互作用和调节可能在铁锈树叶提取物的氧化和抗肿瘤活性中起重要作用。
