Jew J Y, Williams T H, Gabella G, Zhang M Q
Department of Anatomy, University of Iowa College of Medicine, Iowa City.
Arch Histol Cytol. 1989;52 Suppl:167-80. doi: 10.1679/aohc.52.suppl_167.
This study focuses on establishing the nature and extent of the changes that occur in gastroenteric innervation, specifically the myenteric plexus of the rat ileum, following an injury generated by experimental obstruction. A partial obstruction was accomplished by placing a cuff around the terminal portion of the ileum of the rat. Substantial hypertrophy of the enteric muscle wall occurred after 3-5 weeks. Light microscopic examination of the myenteric plexus revealed changes in the numbers of neuronal perikarya, ganglia and perikarya per ganglia; sizes and shapes of perikarya; and thicknesses of nerve fiber bundles. Using vasoactive intestinal polypeptide (VIP) and substance P light microscopic immunohistochemistry, we observed indications of transmitter accumulation in cell bodies and nerve fibers and reactive, degenerative and regenerative changes in axonal endings. Electron microscopic studies provided evidence for neuroplastic changes, as demonstrated by the appearance of reactive and regenerative, or growth, cones in the myenteric plexus.
本研究着重于确定在实验性梗阻造成损伤后，胃肠神经支配，特别是大鼠回肠肌间神经丛中所发生变化的性质和程度。通过在大鼠回肠末端放置一个套圈实现部分梗阻。3至5周后出现肠肌壁的显著肥大。对肌间神经丛进行光镜检查发现，神经元胞体数量、神经节以及每个神经节中的胞体数量、胞体的大小和形状以及神经纤维束的厚度均发生了变化。利用血管活性肠肽（VIP）和P物质光镜免疫组织化学方法，我们观察到递质在细胞体和神经纤维中积累的迹象，以及轴突末梢的反应性、退行性和再生性变化。电子显微镜研究为神经可塑性变化提供了证据，肌间神经丛中出现的反应性、再生性或生长性锥体现已证明了这一点。
