Yoshie S, Wakasugi C, Teraki Y, Iwanaga T, Fujita T
Department of Oral Anatomy, Nippon Dental University, Niigata, Japan.
Arch Histol Cytol. 1988 Oct;51(4):379-84. doi: 10.1679/aohc.51.379.
The taste buds and their nerves in the guinea pig were immunocytochemically investigated with regard to the localization of spot 35 protein, neuron-specific enolase (NSE), neurofilament protein (NFP), and S-100 protein. The spot 35 protein-like immunoreactivity was confined to slender cells comprising half the number of taste bud cells. NSE-like immunoreactivity was recognized in some taste bud cells as well as nerve fibers both within the taste bud and in the subepithelial connective tissue. The NSE-immunoreactive cells were divided into two cell populations: one weakly and the other intensely immunoreactive. The former cells proved to be spot 35 protein-immunoreactive. Moreover, the cells immunoreactive for both spot 35 protein and NSE were frequently associated with nerve fibers immunostained intensely for NSE. The NFP- and S-100 protein-like immunoreactivities were found in none of cells in the taste bud, but exclusively in the subepithelial neural elements.
利用免疫细胞化学方法，研究了豚鼠味蕾及其神经中斑点35蛋白、神经元特异性烯醇化酶（NSE）、神经丝蛋白（NFP）和S-100蛋白的定位。斑点35蛋白样免疫反应局限于构成味蕾细胞数量一半的细长细胞。在一些味蕾细胞以及味蕾内和上皮下结缔组织中的神经纤维中都可识别出NSE样免疫反应。NSE免疫反应细胞分为两个细胞群：一个免疫反应弱，另一个免疫反应强。前者细胞被证明具有斑点35蛋白免疫反应性。此外，对斑点35蛋白和NSE均呈免疫反应的细胞常与对NSE免疫染色强的神经纤维相关。在味蕾的细胞中未发现NFP和S-100蛋白样免疫反应，仅在上皮下神经成分中发现。
