Silvestrini B, Guglielmotti A, Saso L, Cheng C Y
Institute of Pharmacology and Pharmacognosy, University of Rome, Italy.
Clin Chem. 1989 Nov;35(11):2207-11.
Quantitative changes of concanavalin A (Con A)-reactive proteins in serum samples obtained from rats with induced inflammation and from patients with inflammatory and autoimmune diseases were examined by use of lectin blots. Treatment of rats with a single dose of fermented yeast to induce inflammation caused an extensive increase in Con A-reactivity. These changes were time dependent and were similar in both sexes of the animals. When we examined serum samples obtained from patients with various inflammatory disorders for their Con A-reactive proteins as compared with normal donors, we noted that the Con A-reactivity increased in patients with rheumatoid arthritis and systemic lupus erythematosus. Among all the glycoproteins examined by lectin blots with use of Con A, a set of five proteins was selected for detailed analysis by densitometric scanning. These included alpha 2-macroglobulin, P-150, P-95, P-40, and P-35, of Mr 180,000, 150,000, 95,000, 40,000, and 35,000, respectively, by sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions. Densitometric scanning analysis of the lectin blots revealed that the Con A-reactivity of these proteins increased during inflammation. Because alpha 2-macroglobulin is not an acute-phase protein in humans, an increase in Con A staining of this protein suggested that altered glycation is associated with autoimmune diseases. Thus, study of changes in Con A-reactive proteins in human sera may facilitate our understanding of the etiology and pathophysiology of autoimmune diseases.
利用凝集素印迹法检测了从诱导炎症的大鼠以及患有炎症和自身免疫性疾病的患者获取的血清样本中伴刀豆球蛋白A（Con A）反应性蛋白的定量变化。用单剂量发酵酵母处理大鼠以诱导炎症，导致Con A反应性大幅增加。这些变化具有时间依赖性，且在动物的雌雄两性中相似。当我们将从各种炎症性疾病患者获取的血清样本与正常供体的样本进行比较，检测其Con A反应性蛋白时，我们注意到类风湿性关节炎和系统性红斑狼疮患者的Con A反应性增加。在用Con A进行凝集素印迹检测的所有糖蛋白中，选择了一组五种蛋白质通过光密度扫描进行详细分析。在还原条件下通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳分析，这些蛋白质分别为α2 - 巨球蛋白、P - 150、P - 95、P - 40和P - 35，其分子量分别为180,000、150,000、95,000、40,000和35,000。凝集素印迹的光密度扫描分析显示，这些蛋白质的Con A反应性在炎症期间增加。由于α2 - 巨球蛋白在人类中不是急性期蛋白，该蛋白Con A染色增加表明糖基化改变与自身免疫性疾病有关。因此，研究人血清中Con A反应性蛋白的变化可能有助于我们理解自身免疫性疾病的病因和病理生理学。
