Saso L, Valentini G, Giardino A M, Spadaro A, Riccieri V, Zoppini A, Silvestrini B
Institute of Pharmacology and Pharmacognosy, University of Rome La Sapienza, Italy.
Biochem Mol Biol Int. 1998 Dec;46(5):867-75. doi: 10.1080/15216549800204412.
Changes of glycosylation of serum proteins of patients with psoriatic arthritis were detected by lectin blotting and a new enzyme-linked lectin assay (ELLA) using concanavalin A (Con A). A good linear correlation was found between the total Con A-reactivity of serum and the serum levels of C-reactive protein and interleukin-6, which is known to regulate the glycosylation pattern of proteins upon inflammation. A good linear correlation was also observed between the immunoreactivity of alpha 1-antitrypsin, measured by ELISA, using a monoclonal antibody sensitive to glycosylation changes, and the erythrocyte sedimentation rate and the serum concentrations of soluble interleukin-2 receptor, an index of lymphocyte activation which correlated with some inflammatory parameters of disease activity. These protein changes, which are described here for the first time, deserve to be studied in further detail in view of their possible clinical applications.
采用伴刀豆球蛋白A（Con A），通过凝集素印迹法和一种新的酶联凝集素测定法（ELLA）检测银屑病关节炎患者血清蛋白的糖基化变化。血清的总Con A反应性与C反应蛋白及白细胞介素-6的血清水平之间存在良好的线性相关性，已知白细胞介素-6在炎症时可调节蛋白质的糖基化模式。使用对糖基化变化敏感的单克隆抗体通过ELISA测定的α1-抗胰蛋白酶的免疫反应性，与红细胞沉降率以及可溶性白细胞介素-2受体的血清浓度之间也观察到良好的线性相关性，可溶性白细胞介素-2受体是淋巴细胞活化的指标，与疾病活动的一些炎症参数相关。本文首次描述的这些蛋白质变化，鉴于其可能的临床应用价值，值得进一步深入研究。
