Al-Qattan Mohammad M, Abd-Al Wahed Mervat M, Hawary Khalid, Alhumidi Ahmed A, Shier Medhat K
Department of Surgery, King Saud University, PO Box 18097, Riyadh 11415, Saudi Arabia ; College of Medicine Research Center, King Saud University, PO Box 18097, Riyadh 11415, Saudi Arabia.
College of Medicine Research Center, King Saud University, PO Box 18097, Riyadh 11415, Saudi Arabia.
Biomed Res Int. 2014;2014:121098. doi: 10.1155/2014/121098. Epub 2014 Mar 30.
nAG (newt-Anterrior Gradient) protein is the key mediator of regrowth of amputated limbs in salamanders. In a previous work in our lab, a new nAG gene (suitable for humans) was designed and cloned. The cloned vector was transfected into primary human fibroblasts. The expression of nAG in human primary fibroblasts was found to suppress collagen expression. The current study shows that local injection of recombinant nAG reduces scar hypertrophy in the rabbit ear model. This is associated with lower scar elevation index (SEI), lower levels of collagen I & III, higher levels of MMP1, and a higher degree of scar maturation in experimental wounds compared to controls.
nAG（蝾螈前梯度）蛋白是蝾螈断肢再生的关键介质。在我们实验室之前的一项工作中，设计并克隆了一个新的nAG基因（适用于人类）。将克隆载体转染到原代人成纤维细胞中。发现人原代成纤维细胞中nAG的表达可抑制胶原蛋白的表达。当前研究表明，在兔耳模型中局部注射重组nAG可减少瘢痕增生。与对照组相比，这与较低的瘢痕隆起指数（SEI）、较低水平的I型和III型胶原蛋白、较高水平的基质金属蛋白酶1以及实验伤口中较高程度的瘢痕成熟有关。
