Pace N R, Smith D K, Olsen G J, James B D
Department of Biology, Indiana University, Bloomington 47405.
Gene. 1989 Oct 15;82(1):65-75. doi: 10.1016/0378-1119(89)90031-0.
The most incisive a priori approach to inferring the higher order structure of large RNAs has proven to be the use of phylogenetic comparisons. This article provides guidelines to the method, using as an illustration the elucidation of the secondary structure of the catalytic RNA subunit of ribonuclease P (RNase P). The resultant structure is compared to the possibilities that are predicted thermodynamically for the RNase P RNA sequences of nine eubacteria.
事实证明，推断大型RNA高阶结构最具洞察力的先验方法是进行系统发育比较。本文提供了该方法的指导方针，并以阐明核糖核酸酶P（RNase P）催化RNA亚基的二级结构为例进行说明。将所得结构与对9种真细菌的RNase P RNA序列进行热力学预测的可能性进行比较。
