Gruppi A, Gea S, Moretti E R, Vottero-Cima E
Cátedra de Inmnología y Serología, Facultade de Ciencias Químicas,Universidad Nacional de Córdoba, Argentina.
Int Arch Allergy Appl Immunol. 1989;90(2):119-23. doi: 10.1159/000235012.
This work describes the occurrence of antibodies against exoantigens of Trypanosoma cruzi in a high percentage of patients with chronic Chagas' disease. When analyzing selected groups of patients, it was observed that sera from individuals with electrocardiographic alterations showed a greater number of precipitin system and higher antibody titers than sera from patients with positive serology only. Partial characterization of exoantigens of T. cruzi was performed by isoelectric focusing and immunoblotting. Two distinct groups of antigens at pI around 5 and 6, respectively, were identified by these methods. It was also shown in absorption experiments that exoantigens of lower pI share epitopes with components of the cellular surface of epimastigotes of T. cruzi, whereas exoantigens of higher pI share epitopes with normal human heart tissue.
这项研究描述了在高比例的慢性恰加斯病患者中存在针对克氏锥虫外抗原的抗体。在分析选定的患者群体时发现，心电图有改变的个体血清中沉淀素系统数量更多，抗体滴度也高于仅血清学呈阳性的患者。通过等电聚焦和免疫印迹对克氏锥虫外抗原进行了部分特性分析。通过这些方法分别鉴定出两组不同的抗原，其等电点分别约为5和6。吸收实验还表明，较低等电点的外抗原与克氏锥虫前鞭毛体细胞表面成分具有共同表位，而较高等电点的外抗原与正常人心脏组织具有共同表位。
