Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan; Organization of Advanced Science and Technology, Kobe University, Kobe, Japan.
Department of Oceanography, National Sun Yat-Sen University, Kaohsiung 804, Taiwan; Asia-Pacific Ocean Research Center, National Sun Yat-Sen University, Kaohsiung 804, Taiwan.
Bioresour Technol. 2014 Jul;163:128-35. doi: 10.1016/j.biortech.2014.04.028. Epub 2014 Apr 19.
A recently isolated thermotolerant microalga Desmodesmus sp. F2 has the traits of becoming potential biodiesel feedstock, such as high growth rate, high lipid content, and quick precipitation. Its overall lipid productivity was 113 mg/L/d when grown under non-optimal conditions using batch cultivation. A two-step response surface methodology was adopted to optimize its cultivation conditions. The overall lipid productivity was increased to 263 mg/L/d when the cells were grown under the optimized conditions of 6.6mM initial nitrogen level and 6 days nitrogen depletion treatment in 700 μmol/m(2)/s light intensity at 35°C using batch cultivation. Fed-batch and semi-continuous cultivations were employed to further increase its lipid productivity to 213 and 302 mg/L/d, respectively. The 302 mg/L/d is the highest overall lipid productivity of microalgae ever reported in the literature. This study provides the information required for the design and operation of photobioreactors for large scale outdoor cultivation of this species.
最近分离出的耐热微藻 Desmodesmus sp. F2 具有成为潜在生物柴油原料的特性，如生长速度快、脂质含量高、沉淀迅速。在分批培养中，在非最适条件下生长时，其总脂生产力达到 113mg/L/d。采用两步响应面法优化其培养条件。当细胞在初始氮水平为 6.6mM 和 6 天氮耗尽处理的优化条件下，在 35°C 、700μmol/m(2)/s 光强下生长时，总脂生产力提高到 263mg/L/d。采用分批补料和半连续培养进一步将其脂产量提高到 213 和 302mg/L/d。302mg/L/d 是文献中报道的微藻总脂生产力的最高值。该研究为该物种的大规模户外培养提供了设计和运行光生物反应器所需的信息。
