皮肤中α和β维甲酸受体的表达。

Expression of the alpha and beta retinoic acid receptors in skin.

作者信息

Rees J L, Redfern C P

机构信息

Department of Dermatology, Medical School, University of Newcastle, Newcastle upon Tyne, United Kingdom.

出版信息

J Invest Dermatol. 1989 Dec;93(6):818-20. doi: 10.1111/1523-1747.ep12284435.

DOI:10.1111/1523-1747.ep12284435

PMID:2479696

Abstract

Retinoic acid receptor (RAR) -alpha and -beta transcripts are expressed in rat and human skin, and in rat and human dermal fibroblasts and keratinocytes in vitro. RAR-alpha transcripts (ca. 2.8 and 3.6 kb) were expressed in all tissues but were more abundant in dermis and dermal fibroblasts than in epidermis or keratinocytes. RAR-beta mRNA was expressed in skin, but patterns of expression differed between human and neonatal rat samples. In human dermal fibroblasts, keratinocytes and whole skin, two RAR-beta transcripts (ca. 3.1 and 3.4 kb) were expressed. Conversely, in neonatal rat skin, dermal fibroblasts, and keratinocytes only the smaller transcript was detectable and was more abundant in cultured cells than in whole tissue. These results suggest that retinoic acid may have complex, as yet undefined, RAR-mediated regulatory functions in both dermis and epidermis.

摘要

维甲酸受体（RAR）α和β转录本在大鼠和人类皮肤中表达，也在体外培养的大鼠和人类真皮成纤维细胞及角质形成细胞中表达。RARα转录本（约2.8和3.6 kb）在所有组织中均有表达，但在真皮和真皮成纤维细胞中比在表皮或角质形成细胞中更为丰富。RARβ mRNA在皮肤中表达，但人类和新生大鼠样本的表达模式有所不同。在人类真皮成纤维细胞、角质形成细胞和全皮中，表达两种RARβ转录本（约3.1和3.4 kb）。相反，在新生大鼠皮肤、真皮成纤维细胞和角质形成细胞中，仅可检测到较小的转录本，且在培养细胞中比在全组织中更为丰富。这些结果表明，维甲酸在真皮和表皮中可能具有复杂的、尚未明确的RAR介导的调节功能。