Motyka Kamil, Stýskala Jakub, Cankař Petr, Hlaváč Jan
Department of Organic Chemistry, Faculty of Science, Institute of Molecular and Translational Medicine, Palacký University, 17. Listopadu 12, 771 46, Olomouc, Czech Republic,
J Fluoresc. 2014 Jul;24(4):1177-82. doi: 10.1007/s10895-014-1398-8. Epub 2014 May 6.
The fluorescence properties of selected benzo[c]phenanthridines (BPs) were examined. The effect of structure, pH and solvent on the fluorescence properties has been investigated. It was found out that the presence of charged iminium nitrogen significantly decreased the fluorescence of the compounds. The fluorescence (intensity as well as emission spectra shape) of the investigated compounds was significantly dependent on pH as well as used solvent. The utilization in epigenetic modification mechanisms studies as demethylase probe and as possible pH indicator was suggested.
研究了所选苯并[c]菲啶（BPs）的荧光性质。研究了结构、pH值和溶剂对荧光性质的影响。结果发现，带电荷的亚胺氮的存在显著降低了化合物的荧光。所研究化合物的荧光（强度以及发射光谱形状）显著依赖于pH值和所用溶剂。建议将其用于表观遗传修饰机制研究中作为去甲基化酶探针以及作为可能的pH指示剂。
