Liu Hao, Wang Jihua, Zhao Jianglin, Lu Shiqiong, Wang Jingguo, Jiang Weibo, Ma Zhanhong, Zhou Ligang
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
Nat Prod Commun. 2009 Nov;4(11):1557-60.
Bioassay-guided fractionation of the crude extract of the whole plant of Macleaya cordata R. Br. led to the isolation of four alkaloids, which were identified as sanguinarine (1), chelerythrine (2), protopine (3) and alpha-allocryptopine (4) on the basis of their physicochemical and spectrometric data. Compounds 1 and 2 demonstrated a significant antifungal activity against the six test fungi with median inhibitory concentrations (IC50) ranging from 0.47 to 6.13 microg/mL. Compound 1 was the most effective with an IC50 of 0.47 microg/mL on Rhizoctonia solani. Furthermore, compounds 1 and 2 also demonstrated strong antibacterial activity, with IC50 values ranging from 5.01 to 11.3 microg/mL, and minimum inhibitory concentrations (MIC) ranging from 8.0 to 32.0 microg/mL. This is the first report on the activity of the alkaloids from M. cordata against economically important phytopathogenic fungi and bacteria.
对博落回全草粗提物进行生物测定导向的分离，得到了四种生物碱，根据其理化和光谱数据鉴定为血根碱（1）、白屈菜红碱（2）、原阿片碱（3）和α-别隐品碱（4）。化合物1和2对六种受试真菌表现出显著的抗真菌活性，中位抑制浓度（IC50）范围为0.47至6.13微克/毫升。化合物1对茄丝核菌最有效，IC50为0.47微克/毫升。此外，化合物1和2还表现出较强的抗菌活性，IC50值范围为5.01至11.3微克/毫升，最低抑菌浓度（MIC）范围为8.0至32.0微克/毫升。这是关于博落回生物碱对重要经济植物病原真菌和细菌活性的首次报道。
