Spagnol G, Williams M, Srinivasan J, Golier J, Bauer D, Lebo R V, Latov N
Department of Neurology, College of Physician and Surgeons, Columbia Presbyterian Medical Center, New York, NY 10032.
J Neurosci Res. 1989 Oct;24(2):137-42. doi: 10.1002/jnr.490240203.
The nucleotide sequence for human myelin-associated glycoprotein (MAG) and its deduced amino acid sequence, obtained by analysis of two overlapping cDNA clones isolated from a human brain cDNA library, is presented and compared to that reported for rat MAG. The sequence provides an open reading frame of 1,878 nucleotides encoding a peptide of 626 amino acids with a calculated molecular weight of 69.1 kD. It is 89% homologous in nucleotide sequence to the large isoform of rat MAG, with 95% homology in the amino acid sequence. It contains 9 potential glycosylation sites, one more than in rat, and shares other key features with rat MAG, including 5 immunoglobulin-like regions of internal homology, an RGD sequence, and potential phosphorylation sites. Its structure appears to be highly conserved in evolution, possibly suggesting a close interdependence between its structure and function. The human gene is located on the proximal long arm of chromosome 19 (19q12----q13.2).
本文展示了通过分析从人脑cDNA文库中分离出的两个重叠cDNA克隆所获得的人髓鞘相关糖蛋白（MAG）的核苷酸序列及其推导的氨基酸序列，并与报道的大鼠MAG序列进行了比较。该序列提供了一个1878个核苷酸的开放阅读框，编码一个626个氨基酸的肽，计算分子量为69.1kD。其核苷酸序列与大鼠MAG的大同工型具有89%的同源性，氨基酸序列同源性为95%。它含有9个潜在的糖基化位点，比大鼠多一个，并且与大鼠MAG具有其他关键特征，包括5个内部同源的免疫球蛋白样区域、一个RGD序列和潜在的磷酸化位点。其结构在进化过程中似乎高度保守，这可能表明其结构与功能之间存在密切的相互依存关系。人类基因位于19号染色体长臂近端（19q12----q13.2）。
