Australian Institute of Marine Science , PMB no. 3, Townsville MC, Townsville, 4810, Australia.
J Nat Prod. 2014 May 23;77(5):1193-200. doi: 10.1021/np500059h. Epub 2014 May 5.
Two species of red algae belonging to the genus Plocamium, P. hamatum from Moreton Bay, Queensland, and P. costatum, from Pandalowie Bay, South Australia, were investigated to assess their chemical variation and as potential sources of new halogenated monoterpenes. The hyphenated technique HPLC-UV-MS-SPE-NMR was used to assess the algal extracts and to determine its potential for accelerated identification of halogenated monoterpenes generally. A combination of the hyphenated and traditional chromatographic techniques resulted in the isolation and characterization of a total of 10 halogenated monoterpene metabolites, eight of which are reported for the first time. Their structures, including configurations, were determined through interpretation of their 1D and 2D NMR, mass spectrometric, infrared, and X-ray data. The two species of Plocamium produced different secondary metabolites and contained a significant number of new polyhalogenated monoterpenes. The investigation also showed the hyphenated technique HPLC-UV-MS-SPE-NMR to be useful for preliminary investigation of the chemical content of algal extracts.
两种属于 Plocamium 属的红藻，即来自昆士兰州莫尔顿湾的 P. hamatum 和来自南澳大利亚州潘达洛威湾的 P. costatum，被调查以评估它们的化学变异情况，并作为新卤代单萜的潜在来源。采用 HPLC-UV-MS-SPE-NMR 技术联用评估藻类提取物，并确定其加速鉴定卤代单萜的潜力。该联用技术与传统色谱技术的结合，成功分离和鉴定了总共 10 种卤代单萜代谢物，其中 8 种为首次报道。通过对它们的 1D 和 2D NMR、质谱、红外和 X 射线数据的解释，确定了它们的结构，包括构型。这两种 Plocamium 产生了不同的次生代谢产物，含有大量新的多卤代单萜。研究还表明，HPLC-UV-MS-SPE-NMR 联用技术可用于初步研究藻类提取物的化学含量。
