Brunner D, Pedersen N C
Department of Medicine, School of Veterinary Medicine, University of California, Davis 95616.
J Virol. 1989 Dec;63(12):5483-8. doi: 10.1128/JVI.63.12.5483-5488.1989.
Macrophages were harvested from the peritoneal cavities of healthy specific-pathogen-free cats by saline lavage. Three days before collection, the peritoneal cavities were stimulated with glutaraldehyde-fixed Saccharomyces cerevisiae cells to induce greater numbers of macrophages and to begin the activation sequence. Peritoneal macrophages from cats stimulated once with yeast consisted mainly of small macrophages and a smaller number of larger activated macrophages. After several days in culture, many of the small macrophages became activated and a portion of the activated macrophages developed into multinucleated giant cells. Peritoneal cells from cats that were stimulated twice or three times with yeast at 3-week intervals consisted of a higher proportion of activated macrophages initially and produced more and larger multinuclear giant cells with time. Cultures of peritoneal cells stimulated once with yeast were easily infected in vitro with feline immunodeficiency virus (FIV) and produced a transient burst of reverse transcriptase activity. After the initial burst of virus replication, the infection became latent. Even more multinucleated giant cells appeared after infection, and many of these cells fused with each other. Replicating virus could be rescued from the latently infected macrophages after 2 to 3 weeks of phorbol myristate acetate stimulation and cocultivation with T-lymphocyte-enriched peripheral blood mononuclear cells. Multiply stimulated peritoneal cells, which contained a much higher proportion of activated macrophages, could also be infected in vitro with FIV. The infection usually became latent, however, without going through an initial replicative stage. Peritoneal cells from chronically FIV-infected specific-pathogen-free cats contained a higher proportion of activated macrophages and were latently infected with FIV from the outset.
通过生理盐水灌洗从健康无特定病原体猫的腹腔中收获巨噬细胞。在采集前三天，用戊二醛固定的酿酒酵母细胞刺激腹腔，以诱导产生更多数量的巨噬细胞并启动激活过程。用酵母单次刺激的猫的腹腔巨噬细胞主要由小巨噬细胞和少量较大的活化巨噬细胞组成。培养几天后，许多小巨噬细胞被激活，一部分活化巨噬细胞发育成多核巨细胞。每隔3周用酵母刺激两次或三次的猫的腹腔细胞最初含有较高比例的活化巨噬细胞，并且随着时间的推移产生更多更大的多核巨细胞。用酵母单次刺激的腹腔细胞培养物在体外很容易被猫免疫缺陷病毒（FIV）感染，并产生短暂的逆转录酶活性爆发。在病毒复制的初始爆发后，感染变为潜伏状态。感染后出现更多的多核巨细胞，其中许多细胞相互融合。在用佛波酯肉豆蔻酸酯刺激2至3周并与富含T淋巴细胞的外周血单核细胞共培养后，可以从潜伏感染的巨噬细胞中拯救出复制病毒。多次刺激的腹腔细胞含有更高比例的活化巨噬细胞，也可以在体外被FIV感染。然而，感染通常会变为潜伏状态，而不经过初始复制阶段。来自慢性FIV感染的无特定病原体猫的腹腔细胞含有更高比例的活化巨噬细胞，并且从一开始就被FIV潜伏感染。
