Department of Neurology, Charité - Universitätsmedizin Berlin, Germany; Clinical and Experimental Multiple Sclerosis Research Center, Charité - Universitätsmedizin Berlin, Germany.
Department of Neurology, Charité - Universitätsmedizin Berlin, Germany; Clinical and Experimental Multiple Sclerosis Research Center, Charité - Universitätsmedizin Berlin, Germany.
J Neuroimmunol. 2014 Jul 15;272(1-2):56-61. doi: 10.1016/j.jneuroim.2014.04.005. Epub 2014 Apr 16.
Patients with multiple sclerosis (MS) have elevated antibodies against Epstein-Barr virus (EBV), but data on the epitope-resolved specificity of these antibodies are scarce. Using a peptide microarray containing 1465 peptides representing 8 full-length EBV proteins, we identified higher (p<0.001) antibody reactivities to 39 EBV-peptides in MS patients (n=29) compared to healthy controls (n=22). Seventeen of the 39 peptides were from EBNA-1 and 13 located within the glycine-alanine repeat of EBNA-1. Further reactivities were directed against EBNA-3, EBNA-4, EBNA-6, VP26, and LMP1. Thus, antibodies against EBV in MS patients primarily target, but are not confined to, the glycine-alanine repeat of EBNA-1.
多发性硬化症（MS）患者体内存在针对 Epstein-Barr 病毒（EBV）的抗体升高，但针对这些抗体的表位解析特异性的数据却很少。我们使用包含 1465 个代表 8 种全长 EBV 蛋白的肽的肽微阵列，发现与健康对照组（n=22）相比，MS 患者（n=29）对 39 种 EBV 肽的抗体反应更高（p<0.001）。39 种肽中有 17 种来自 EBNA-1，13 种位于 EBNA-1 的甘氨酸-丙氨酸重复区。进一步的反应性针对 EBNA-3、EBNA-4、EBNA-6、VP26 和 LMP1。因此，MS 患者体内针对 EBV 的抗体主要针对但不限于 EBNA-1 的甘氨酸-丙氨酸重复区。
