Lavoué R, Geffré A, Braun J P, Peeters D, Granat F, Bourgès-Abella N, Trumel C
Internal Medicine Unit , Institut National Polytechnique, Ecole Nationale Vétérinaire de Toulouse (INP-ENVT), Toulouse, France.
Vet Clin Pathol. 2014 Sep;43(3):352-61. doi: 10.1111/vcp.12151. Epub 2014 May 5.
There is an increasing interest for breed-specific reference intervals in veterinary medicine. In a previous study, breed-specific biochemical reference intervals (RIs) have been established for Dogues de Bordeaux (DDBs). This breed is predisposed to familial juvenile glomerulonephropathy and hypothyroidism, and would benefit from hematologic RI.
The purpose was de novo establishment of breed-specific hematologic RIs for the DDB in accordance with the International Federation of Clinical Chemistry and Clinical and Laboratory Standards Institute guidelines.
One hundred and twenty DDBs from France and Belgium were recruited. CBCs were determined with the Sysmex XT-2000iV analyzer within 12 hours of blood collection. RIs were determined using the nonparametric method. Effects of sex, age, and face mask color were studied.
RIs were determined in 58 healthy dogs. DDBs had higher RIs for HGB, HCT, MCV, MCHC, and mean platelet volume, and lower RIs for reticulocytes counts, platelets by impedance (PLT-I) and optical count (PLT-O), and plateletcrit when compared with generic canine RIs. Age significantly affected RIs for HGB, HCT, MCHC, WBC, neutrophil, lymphocyte, and monocyte counts.
The generic canine RIs established in the same laboratory with analogous preanalytical and analytical variations did not differ significantly from breed-specific RIs, and thus have no significant impact on clinical decision making; however, breed-specific RIs are advised for some RBC and all platelet-related variables to avoid erroneous suspicion of polycythemia and thrombocytopenia when using general canine RIs for evaluation of DDB.
兽医学中对特定品种参考区间的关注日益增加。在之前的一项研究中，已经为波尔多犬（DDBs）建立了特定品种的生化参考区间（RIs）。该品种易患家族性幼年肾小球肾炎和甲状腺功能减退症，并且将从血液学RI中受益。
目的是根据国际临床化学和临床与实验室标准协会指南重新建立DDB的特定品种血液学RIs。
招募了来自法国和比利时的120只DDB。在采血后12小时内使用Sysmex XT-2000iV分析仪测定全血细胞计数（CBCs）。使用非参数方法确定RIs。研究了性别、年龄和面罩颜色的影响。
在58只健康犬中确定了RIs。与一般犬类RIs相比，DDB的血红蛋白（HGB）、血细胞比容（HCT）、平均红细胞体积（MCV）、平均红细胞血红蛋白浓度（MCHC）和平均血小板体积的RIs较高，而网织红细胞计数、阻抗法血小板计数（PLT-I）、光学法血小板计数（PLT-O）和血小板压积的RIs较低。年龄显著影响HGB、HCT、MCHC、白细胞（WBC）、中性粒细胞、淋巴细胞和单核细胞计数的RIs。
在同一实验室建立的具有类似分析前和分析差异的一般犬类RIs与特定品种RIs没有显著差异，因此对临床决策没有显著影响；然而，对于某些红细胞和所有血小板相关变量，建议使用特定品种RIs，以避免在使用一般犬类RIs评估DDB时错误地怀疑红细胞增多症和血小板减少症。
