Serra Mariana, Freeman Kathleen P, Campora Carola, Sacchini Federico
IDEXX Laboratories Wetherby, Wetherby, West Yorkshire, UK.
Vet Clin Pathol. 2012 Jun;41(2):207-15. doi: 10.1111/j.1939-165X.2012.00417.x. Epub 2012 Mar 5.
The Sysmex XT-2000iV is a hematology analyzer that combines impedance and optical techniques and has been previously validated for dogs. Specific reference intervals (RIs) are useful when interpreting results.
The aim of this study was to determine hematologic RIs for the Sysmex XT-2000iV using a large reference population of client-owned clinically healthy blood donor dogs, adopting an indirect sampling method.
Dogs were screened for breed, size, health, travel history, and previous blood transfusions, and the quality of blood specimens was also reviewed. Results from specimens that met inclusion criteria were used to determine RIs using a nonparametric method. Specimens from Akitas and sighthounds were excluded from the study.
Of 992 specimens that had been collected from blood donors and analyzed, 297 were initially included in the RI study. An additional 38 specimens were excluded as outliers, and hematologic RIs for the Sysmex XT-2000iV were based on analysis of specimens from 259 clinically healthy dogs. Measurands evaluated had variable distributions, and intervals obtained were generally comparable to previously reported RIs. Differences observed included higher lower and upper reference limits (LRL and URL, respectively) for MCV and lower URL for WBC count. Reticulocyte count and the LRL of the absolute lymphocyte count were also higher than previously reported, and the RI for platelet count was narrower and lower.
Canine RIs for the Sysmex hematology analyzer were established using an indirect sampling method with reference individuals selected from a large database of client-owned clinically healthy blood donor dogs. For specimens included in this study, time from collection to analysis was similar to what veterinary commercial laboratories experience.
Sysmex XT - 2000iV是一款结合了阻抗和光学技术的血液分析仪，此前已在犬类中得到验证。特定的参考区间（RIs）在解释结果时很有用。
本研究的目的是采用间接抽样方法，使用大量客户拥有的临床健康献血犬的参考群体，确定Sysmex XT - 2000iV的血液学参考区间。
对犬只进行品种、体型、健康状况、旅行史和既往输血史的筛查，并对血液标本的质量进行评估。符合纳入标准的标本结果用于采用非参数方法确定参考区间。秋田犬和视觉猎犬的标本被排除在研究之外。
在从献血犬采集并分析的992份标本中，最初有297份被纳入参考区间研究。另外38份标本作为异常值被排除，Sysmex XT - 2000iV的血液学参考区间基于对259只临床健康犬的标本分析。所评估的测量指标具有不同的分布，获得的区间总体上与先前报道的参考区间相当。观察到的差异包括平均红细胞体积（MCV）的较低和较高参考限值（分别为LRL和URL）较高，白细胞计数的较高参考限值较低。网织红细胞计数和绝对淋巴细胞计数的较低参考限值也高于先前报道，血小板计数的参考区间更窄且更低。
Sysmex血液分析仪的犬类参考区间是采用间接抽样方法建立的，参考个体选自大量客户拥有的临床健康献血犬数据库。对于本研究中纳入的标本，从采集到分析的时间与兽医商业实验室的经验相似。
