Zennaro E, Francisci S, Ragnini A, Frontali L, Bolotin-Fukuhara M
Department of Cell and Developmental Biology, University of Rome, Italy.
Nucleic Acids Res. 1989 Jul 25;17(14):5751-64. doi: 10.1093/nar/17.14.5751.
A temperature sensitive mutation mapping in the tRNA region of the mitochondrial genome of S. cerevisiae has been found to abolish 3' processing of tRNA(asp). Mutant cells grown for a few generations at the non-permissive temperature were found to specifically lack mature tRNA(asp) and to accumulate 3' unprocessed precursors of this tRNA. The accumulation of precursors of other mitochondrial tRNAs was also observed under the same conditions. After longer incubation times, a generalized decrease of mitochondrial transcripts could be observed. The mutation was genetically mapped in a limited region surrounding the tRNA(asp) gene and found, by sequencing, to consist of a C- greater than T transition at position 61 of the tRNA(asp) gene.
已发现酿酒酵母线粒体基因组tRNA区域中的一个温度敏感突变会消除tRNA（asp）的3'加工。在非允许温度下培养几代的突变细胞被发现特异性缺乏成熟的tRNA（asp），并积累该tRNA的3'未加工前体。在相同条件下也观察到其他线粒体tRNA前体的积累。孵育时间延长后，可观察到线粒体转录本普遍减少。该突变在tRNA（asp）基因周围的有限区域进行遗传定位，通过测序发现它由tRNA（asp）基因第61位的C大于T的转换组成。
