Shevchenko Valeriy E, Kovalev Sergey V, Arnotskaya Natalia E, Kudryavtsev Igor A
Eur J Mass Spectrom (Chichester). 2013;19(5):377-89. doi: 10.1255/ejms.1247.
A label-free nano-liquid chromatography tandem mass spectrometry proteomics analysis on the conditioned media (CM) of two lung cancer cell lines of different histological backgrounds to identify secreted or membrane-bound proteins as novel lung cancer biomarkers was performed. Five hundred and seventy seven proteins were identified and 38% of them were classified as extracellular or membrane-bound. For the search of potential biomarkers of lung cancer a series of selection criteria were proposed. We detected known or putative lung cancer markers. In addition, 40 novel proteins were identified, whose role as biomarkers of lung cancer should be explored further.
对具有不同组织学背景的两种肺癌细胞系的条件培养基（CM）进行了无标记纳米液相色谱串联质谱蛋白质组学分析，以鉴定作为新型肺癌生物标志物的分泌蛋白或膜结合蛋白。共鉴定出577种蛋白质，其中38%被归类为细胞外或膜结合蛋白。为了寻找肺癌的潜在生物标志物，提出了一系列选择标准。我们检测到已知或推测的肺癌标志物。此外，还鉴定出40种新蛋白质，其作为肺癌生物标志物的作用有待进一步探索。
