Vetchinkina Elena P, Loshchinina Ekaterina A, Burov Andrey M, Dykman Lev A, Nikitina Valentina E
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov 410049, Russia.
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov 410049, Russia.
J Biotechnol. 2014 Jul 20;182-183:37-45. doi: 10.1016/j.jbiotec.2014.04.018. Epub 2014 May 4.
We report for the first time that the medicinal basidiomycete Lentinus edodes can reduce Au(III) from chloroauric acid (HAuCl4) to elemental Au [Au(0)], forming nanoparticles. Several methods, including transmission electron microscopy, electron energy loss spectroscopy, X-ray fluorescence, and dynamic light scattering, were used to show that when the fungus was grown submerged, colloidal gold accumulated on the surface of and inside the mycelial hyphae as electron-dense particles mostly spherical in shape, with sizes ranging from 5 to 50nm. Homogeneous proteins (the fungal enzymes laccase, tyrosinase, and Mn-peroxidase) were found for the first time to be involved in the reduction of Au(III) to Au(0) from HAuCl4. A possible mechanism forming Au nanoparticles is discussed.
我们首次报道，药用担子菌香菇能将氯金酸（HAuCl4）中的金（III）还原为元素金[Au(0)]，形成纳米颗粒。我们使用了几种方法，包括透射电子显微镜、电子能量损失谱、X射线荧光和动态光散射，来证明当真菌在液体培养时，胶体金作为电子致密颗粒积累在菌丝体表面和内部，这些颗粒大多呈球形，大小在5到50纳米之间。首次发现同源蛋白（真菌酶漆酶、酪氨酸酶和锰过氧化物酶）参与了将氯金酸中的金（III）还原为金（0）的过程。本文还讨论了形成金纳米颗粒的可能机制。
