State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
FEBS Lett. 2014 Jun 5;588(12):2055-62. doi: 10.1016/j.febslet.2014.04.036. Epub 2014 May 5.
This study aimed to test the exact functions and potential mechanisms of miR-17-5p in gastric cancer. Using real-time PCR, miR-17-5p was found to be expressed more highly in gastric cancer compared with-normal tissues. Gain- and loss-of-function assays demonstrated that miR-17-5p increased the proliferation and growth of gastric cancer cells in vitro and in vivo. Through reporter gene and western blot assays, SOCS6 was shown to be a direct target of miR-17-5p, and proliferative assays confirmed that SOCS6 exerted opposing function to that of miR-17-5p in gastric cancer. In short, miR-17-5p might function as a pro-proliferative factor by repressing SOCS6 in gastric cancer.
本研究旨在探究 miR-17-5p 在胃癌中的具体功能和潜在机制。通过实时 PCR 发现，miR-17-5p 在胃癌组织中的表达水平明显高于正常组织。Gain-和 loss-of-function 实验表明，miR-17-5p 可促进胃癌细胞在体外和体内的增殖和生长。通过报告基因和 Western blot 实验，SOCS6 被证实是 miR-17-5p 的直接靶基因，增殖实验证实 SOCS6 在胃癌中发挥与 miR-17-5p 相反的作用。总之，miR-17-5p 可能通过抑制 SOCS6 在胃癌中发挥促增殖作用。
