Department of General Surgery, First Affiliated Hospital of Jiamusi University, Jiamusi, Heilongjiang 154002, P.R. China.
Department of Interventional Radiology, First Affiliated Hospital of Jiamusi University, Jiamusi, Heilongjiang 154002, P.R. China.
Mol Med Rep. 2021 Nov;24(5). doi: 10.3892/mmr.2021.12411. Epub 2021 Sep 7.
Non‑coding RNAs serve essential roles in regulating mRNA and protein expression and dysregulation of non‑coding RNAs participates in a variety of types of cancer. microRNAs (miRNAs/miRs), which are 21‑24 nucleotides non‑coding RNAs, have been shown to be important for the development of gastric cancer (GC). However, the role of miR‑486‑5p in GC remains to be elucidated. The present study found that miR‑486‑5p was downregulated in GC tissues. Comparing with gastric normal cells GES‑1, GC cells, including MKN‑45, AGS, HGC27 and MKN74, had reduced abundance of miR‑486‑5p transcript. CCK8 and colony formation assays demonstrated that GC cell growth and proliferation were enhanced by miR‑486‑5p inhibitors and were suppressed by miR‑486‑5p mimics. miR‑486‑5p also suppressed cell cycle process and migration and promoted apoptosis in GC cells, as verified by propidium iodide (PI) staining, Transwell assay and PI/Annexin V staining. miR‑486‑5p downregulated fibroblast growth factor 9 (FGF9) through combining to its 3'untranslated region. Overexpression of FGF9 accelerated the growth and proliferation of GC cells. The expression of miR‑486‑5p was negatively associated with FGF9 mRNA expression in GC samples. These results revealed that miR‑486‑5p was a tumor suppressor in GC. Downregulation of FGF9 contributed to the role of miR‑486‑5p in GC.
非编码 RNA 在调节 mRNA 和蛋白质表达方面发挥着重要作用，非编码 RNA 的失调参与了多种类型的癌症。microRNAs（miRNAs/miRs）是 21-24 个核苷酸的非编码 RNA，已被证明对胃癌（GC）的发展很重要。然而，miR-486-5p 在 GC 中的作用仍有待阐明。本研究发现 miR-486-5p 在 GC 组织中下调。与胃正常细胞 GES-1 相比，GC 细胞包括 MKN-45、AGS、HGC27 和 MKN74，miR-486-5p 转录本的丰度降低。CCK8 和集落形成实验表明，miR-486-5p 抑制剂增强了 GC 细胞的生长和增殖，而 miR-486-5p 模拟物则抑制了 GC 细胞的生长和增殖。miR-486-5p 还通过结合其 3'非翻译区来抑制细胞周期过程、迁移并促进 GC 细胞凋亡，这通过碘化丙啶（PI）染色、Transwell 测定和 PI/Annexin V 染色得到证实。miR-486-5p 通过结合其 3'非翻译区下调成纤维细胞生长因子 9（FGF9）。FGF9 的过表达加速了 GC 细胞的生长和增殖。GC 样本中 miR-486-5p 的表达与 FGF9 mRNA 表达呈负相关。这些结果表明 miR-486-5p 是 GC 的肿瘤抑制因子。FGF9 的下调有助于 miR-486-5p 在 GC 中的作用。
