School of Respiratory Therapy, College of Medicine, Taipei Medical University, Taipei, Taiwan; Division of Pulmonary Medicine, Department of Internal Medicine, Shuang Ho Hospital, Taipei Medical University, Taipei, Taiwan.
State Key Laboratory in Marine Pollution-Croucher Institute for Environmental Sciences, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.
J Hazard Mater. 2014 Jun 15;274:384-91. doi: 10.1016/j.jhazmat.2014.04.029. Epub 2014 Apr 21.
The potential effects of inhaled fine particulate matter (PM2.5), found in haze episodes, on the oxidation of the proteins in the lungs are not well understood. We investigated the effects of PM2.5 from haze episodes on protein oxidation. PM2.5 was collected from the air pollution in Beijing (BJ), Xian (XA), Xiamen (XM) and Hong Kong (HK) during a period of intensive haze episodes. The chemical characteristics of these samples and their effects on albumin oxidation were investigated. The levels of PM2.5 in BJ and XA were 4-6 times higher than in XM and HK. The concentrations of the polycyclic aromatic hydrocarbons (PAHs) components of the PM2.5 from BJ and XA were 10 times higher than those found in XM and HK. The haze PM2.5 increased oxidative stress. Addition of PM2.5 samples collected from haze episodes to albumin in vitro resulted in oxidation of methionine moieties; nasal instillation of PM2.5 suspensions in mice resulted in oxidation of methionine in the albumin in the bronchoalveolar lavage fluid. The methionine moieties participate in peptide chain crosslinking, and methionine oxidation in the albumin could be attributed to the PAH compounds. Our findings may be helpful in explaining the potential respiratory effects during haze episodes.
在雾霾期间，吸入的细颗粒物（PM2.5）对肺部蛋白质氧化的潜在影响尚未得到充分理解。我们研究了雾霾期间空气中 PM2.5 对蛋白质氧化的影响。从北京（BJ）、西安（XA）、厦门（XM）和香港（HK）的空气污染中收集 PM2.5 样本，研究了这些样本的化学特性及其对白蛋白氧化的影响。BJ 和 XA 的 PM2.5 水平比 XM 和 HK 高 4-6 倍。BJ 和 XA 的 PM2.5 中多环芳烃（PAHs）成分的浓度比 XM 和 HK 高 10 倍。雾霾 PM2.5 增加了氧化应激。将雾霾期间收集的 PM2.5 样品添加到白蛋白中，会导致甲硫氨酸部分氧化；将 PM2.5 混悬液鼻内滴注到小鼠体内，会导致支气管肺泡灌洗液中白蛋白的甲硫氨酸氧化。甲硫氨酸部分参与肽链交联，白蛋白中的甲硫氨酸氧化可能归因于 PAH 化合物。我们的研究结果可能有助于解释雾霾期间潜在的呼吸道影响。
