Meizel S, Turner K O
J Exp Zool. 1986 Jan;237(1):137-9. doi: 10.1002/jez.1402370118.
The glycosaminoglycans (GAGs) hyaluronic acid and heparin were added (10 micrograms and 100 micrograms/ml to golden hamster sperm suspensions previously incubated for 4.5 h under capacitating conditions. After additions, sperm were incubated for 5-15 min and acrosome reactions (AR) assayed in motile sperm by phase contrast microscopy. Hyaluronic acid and heparin significantly stimulated AR over control levels. Hyaluronic acid did not stimulate AR 15 min after addition to sperm previously incubated for only 2.5 h. Pre-incubation of hyaluronic acid with streptomyces hyaluronidase destroyed the ability of that GAG to stimulate the AR. These results indicate that GAGs (at least one of which, hyaluronic acid, is present in the oocyte cumulus oophorous) can rapidly stimulate the acrosome reaction in motile previously capacitated hamster sperm.
将糖胺聚糖（GAGs）透明质酸和肝素添加到在获能条件下预先孵育4.5小时的金黄仓鼠精子悬液中（浓度分别为10微克/毫升和100微克/毫升）。添加后，精子孵育5 - 15分钟，通过相差显微镜在活动精子中检测顶体反应（AR）。透明质酸和肝素显著刺激AR超过对照水平。在添加到仅预先孵育2.5小时的精子后15分钟，透明质酸未刺激AR。透明质酸与链霉菌透明质酸酶预孵育会破坏该GAG刺激AR的能力。这些结果表明，GAGs（其中至少一种，透明质酸，存在于卵丘卵母细胞中）可快速刺激活动的、先前已获能的仓鼠精子中的顶体反应。
