Domino S E, Garbers D L
Department of Pharmacology, Vanderbilt University Medical Center, Nashville, Tennessee 37232.
Biol Reprod. 1989 Jul;41(1):133-41. doi: 10.1095/biolreprod41.1.133.
The fucose-sulfate glycoconjugate (FSG) component of sea urchin egg jelly that induces an acrosome reaction in spermatozoa-stimulated multiple Ca2+-dependent phospholipid changes in the sperm cell head and flagellum. When cells were radiolabeled with myo-[3H]inositol, FSG treatment decreased radioactivity in phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate within 30 s. In addition, FSG treatment elevated concentrations of phosphatidic acid in spermatozoa. The Ca2+-channel antagonist, verapamil, inhibited the effects of FSG on [3H]polyphosphoinositides and phosphatidic acid. To investigate the possible compartmentalization of phospholipid turnover, isolated heads and flagella were prepared. Treatment of sperm heads with FSG or the monovalent cation ionophore, gramicidin S, caused increased [3H]inositol phosphate and phosphatidic acid accumulation and induction of an acrosome reaction. Effects of FSG and gramicidin S on phosphatidic acid elevations in sperm heads and intact cells were inhibited by verapamil. FSG failed to cause detectable changes in [3H]inositol phosphate or phosphatidic acid concentrations in isolated flagellar preparations. However, when cells were treated with FSG and the flagella were isolated subsequently, phosphatidic acid concentrations in the flagellar preparations were increased.
海胆卵胶膜中的岩藻糖 - 硫酸糖缀合物（FSG）成分可诱导精子发生顶体反应，刺激精子细胞头部和鞭毛中多种依赖钙离子的磷脂变化。当细胞用肌醇 - [³H]肌醇进行放射性标记时，FSG处理在30秒内降低了磷脂酰肌醇4 - 磷酸和磷脂酰肌醇4,5 - 二磷酸中的放射性。此外，FSG处理提高了精子中磷脂酸的浓度。钙离子通道拮抗剂维拉帕米抑制了FSG对[³H]多磷酸肌醇和磷脂酸的影响。为了研究磷脂周转的可能区室化，制备了分离的头部和鞭毛。用FSG或单价阳离子离子载体短杆菌肽S处理精子头部，导致[³H]肌醇磷酸和磷脂酸积累增加，并诱导顶体反应。维拉帕米抑制了FSG和短杆菌肽S对精子头部和完整细胞中磷脂酸升高的影响。FSG未能在分离的鞭毛制剂中引起可检测到的[³H]肌醇磷酸或磷脂酸浓度变化。然而，当细胞用FSG处理并随后分离鞭毛时，鞭毛制剂中的磷脂酸浓度增加。
